Background: Currently, most of the available serological diagnostic kits for strongyloidiasis are based on the use of the crude antigens of Strongyloides ratti, which are good, but with less sensitivity towards the infection. Hence, this study aimed to produce and evaluate monoclonal antibody for detecting soluble parasite antigen in animal sera. Methods: The study was conducted in the Department of Medical Microbiology and Parasitology, University Putra Malaysia in 2014-2017. Saline extract protein from the infective larvae of S. ratti was used to immunize BALB/c mice and subsequent fusion of the B-cells with myeloma cells (SP2/0) using 50% PEG. The hybridomas were cultured in HAT medium and cloned by limiting dilutions. Positive hybrids were screened by indirect ELISA. The ascites fluid from the antibody -secreting hybridoma was purified and the MAb was characterized by western-blots and evaluated in sandwich ELISA for reactivity against the homologous and heterologous antigens. Results: An IgG1 that recognizes a 30 and 34 kDa protein bands was obtained. The MAb was recognized by all S. ratti-related antigens and cross-reacted with only Tomeara cam's antigens in both assays. The minimum antigen detection limit was found to be 5 ng/ml. All antibody-positive rat and dog sera evaluated have shown antigen -positive reactions in Sandwich-ELISA. Conclusion: The MAb produced, was able to detect antigens in strongyloidiasis and toxocariasis in animal models and may also be useful for the serological detection of active strongyloidiasis and visceral toxocariasis in human sera.

Many modern-day diagnostic tests for parasitic diseases rely on conventional labour-intensive technologies such as serology and microscopy. Although major advances have been recorded in the diagnosis of infectious diseases in humans, parasitic diseases continue to present challenges, particularly in resource-poor countries, and this is mainly attributable to war and famine. Factors such as poverty, deteriorated health facilities and destruction of infrastructure are the consequence of the lack of suitable sanitary practices and proper hygiene, especially in refugee camps, that adversely promote infectious diseases to migrants, particularly among vulnerable children. Generally, the gastrointestinal tract is the predilection site for most helminths and protozoa. They are therefore regarded as a serious public-health problem, as they cause malabsorption, malnutrition and blood loss, leading to anaemia or even death. In addition to their health effects, parasitic infections cause physical and mental impairment in children, retard their educational achievements and hinder economic development.