Browsing by Author "Aidil Abdul Hamid"
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Publication Antibacterial Activity of Surfactin Produced by Bacillus subtilis MSH1(Universiti Malaysia Sabah (UMS), 2017) ;Mohd Hafez Mohd Isa ;Mohammed Abdel-Hafiz Faisal Shannaq ;Najwa Mohamed ;Abdul Rahman Hassan ;Najeeb Kaid Nasser Al-ShorganiAidil Abdul HamidSurfactin is one of the most powerful lipopeptide biosurfactants produced by various strains of Bacillus subtilis, having exceptional surface activity as well as antiviral, antibacterial and antitumor properties. In this study, fermentations in shake flasks were conducted to assess the ability of B. subtilis MSH1 to produce surfactin in Cooper's media. Investigation of antibacterial activity of surfactin against Shigella dysenteriae and Staphylococcus aureus by using well diffusion method, minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) shows surfactin having potent bacteriostatic and bactericidal properties which potentially could be utilized for commercial antibiotic formulations with medical and pharmaceutical purposes. - Some of the metrics are blocked by yourconsent settings
Publication Antioxidant Properties And Glucan Compositionof Various Crude Extract From Lentinus Squarrosulus Mycelial Culture(Scientific Research, 2014) ;Rahayu Ahmad ;Sutha Muniandy ;Nur Iffah Abdullah Shukri ;Siti Mariatul Usyida Alias ;Aidil Abdul Hamid ;Wan Mohtar Wan Yusoff ;Sahidan SenafiFauzi DaudVarious crude extracts of Lentinus squarrosulus mushroom mycelium and culture broth were prepared for antioxidant studies, glucan and carbohydrate compound analysis. Crude extract from ethanol, cold and hot water extraction was tested on several complementary test systems namely DPPH free radical scavenging, β-carotene linoleic acid oxidation, total phenolic compound, total flavonoid compound, total carbohydrate content and total glucan compositions. The antioxidant activity of the crude extracts of Lentinus squarrosulus mushroom mycelium and culture broth was determined by 2,2’ diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and β-carotene linoleic acid assay. Crude extracted from culture broth EPS EE recorded the highest radical scavenging effects at 20 mg/mL (85%) which is closer to the control antioxidants (ascorbic acid 97%; BHT 92.4%; BHA 93.6%). Both the ethanol extract (IPS EE and EPS EE) exhibited the highest β-carotene linoleic acid oxidation by recording IC50 values of 0.6 and 0.65 mg/mL respectively. IPS EE (0.59 mg/g) and EPS WE (0.58 mg/g) showed high flavonoid content whereas total phenol content was high in EPS WE (0.85 mg/g) and IPS EE (0.84 mg/g). Total carbohydrate concentrations were high in IPS HWE and IPS CWE with 19.1 and 16.3 mg/mL respectively and these data correlate with the β-glucan content in both extracts. The highest β-glucan content found in IPS CWE and IPS HWE were 12.2 and 11.4% w/w respectively. - Some of the metrics are blocked by yourconsent settings
Publication Production And Antiproliferative Activity Of Various Crude Extract From Lentinus Squarrosulus Mycelium(Scholars Academic and Scientific Publishers, 2015) ;Rahayu Ahmad ;Siti Mariatul Usyida Alias ;Aidil Abdul Hamid ;Wan Mohtar Wan Yusoff ;Endom IsmailFauzi DaudProduction of extracellular and intracellular polysaccharides (EPS, IPS) from Lentinus squarrosulus mycelium was carried out through submerged fermentation. The one factor-at-a-time method was adopted to investigate the effect of medium components (carbon, nitrogen), and environmental factor (initial pH) on dry weight of mycelium and EPS concentration. Sucrose and yeast extract gave the highest EPS concentration with 3.56 mg/mL and 1.23 mg/mL respectively when carbon and nitrogen sources were employed. Combination of ammonium chloride and yeast extract (30:70) ratio increased the mycelium dry weight up to 10.8 g/L and EPS concentration of 4.39 mg/mL. An initial pH of 7.5 showed maximum EPS concentration of 3.82 mg/mL with mycelium dry weight of 7 g/L. Crude extracts from hot water and cold water extraction of Lentinus squarrosulus mycelium (IPS HWE, IPS CWE) and water extract from supernatant (EPS WE) was proved to have antiproliferative activity against A549 lung carcinoma cell lines (ATCC) as compared to ethanol extract. - Some of the metrics are blocked by yourconsent settings
Publication Production of Surfactin from Bacillus subtilis ATCC 21332 by Using Treated Palm Oil Mill Effluent (POME) as Fermentation Media(IACSITPress, Singapore, 2013) ;Mohd Rizal Abas ;Abdul Jalil Abdul Kader ;Mohd Sahaid Khalil ;Aidil Abdul HamidMohd Hafez Mohd IsaSurfactin, a lipopeptidic biosurfactant from Bacillus subtilis can only be produced under appropriate fermentation conditions and one of the factor being considered is their nutrient source. Conventionally, production of surfactin had been practised by utilizing commercial laboratory media in either both small and large scale fermentation. Alternative media options are being sought from agro-based wastes in order to minimize the production cost due to its relatively abundant and inexpensive raw materials. Palm oil mill effluent (POME), an agricultural waste from palm oil industry has been reviewed as a promising candidate that could potentially to be exploited. This study investigated on the feasibility of POME as fermentation media in surfactin production by using prominent surfactin producer of B. subtilis American Type Culture Collection(ATCC) 21332. Nutrient analysis showed POME consisted of significant amount of fermentable sugars, nitrogenous compounds, and essential elements that could support the bacterial growth and surfactin production. Fermentation study evaluated that POME media at various concentrations (10, 30, and 50%) were capable to produce surfactin with different yields. The highest surfactin amount was achieved by using 50 % (v/v) of POME compared to other concentrations studied. Keywords: Surfactin, lipopeptides, Bacillus subtilis ATCC 21332, palm oil mill effluent (POME), fermentation