Trichoderma reesei sp is an anamorph fungi which involves asexual reproduction to form discrete anamorphs in mold like configuration. It also has high rate of mycelium growth of 100% and rate of methylene blue biodegradation of 100% by day 6 in culture medium. Since methylene blue dye is one of the dyes involved in dye pollution, fungi treatment is found as most immaculate compared to other technologies in our current timeline. Agar medium is first-handedly prepared in the laboratory which consists of nutrient agar (20g/L) and glucose (20g/L). At a subsequent time, second culture of agar medium is effectuated by adding methylene blue dye (50mg/L) in agar medium to calculate the rate of methylene blue dye decolourisation before proceeding with the physico-chemical parameters determination in terms of carbon source and nitrogen source for optimum growth in a conducive environment, ascertaining the rate of methylene blue decolourisation. Liquid medium is prepared prior to the investigation of the physico-chemical parameters. By keeping pH 8 and temperature of 37°c constant, the optimum carbon source is determined by contrasting the carbon source concentration (5g/100mL, 10g/100mL, 15g/100mL, 20g/100mL, 25g/100mL, 30g/100mL) corresponding to nitrogen source determination by also contrasting the nitrogen source concentration. Methylene blue decolourisation in optimum conditions is then analysed using UV-vis spectrophotometry.