USIM Research Repository Angular :: Browsing by Author "Anthony, AA"

Browsing by Author "Anthony, AA"

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Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development
(Elsevier Science Inc, 2013)
Ong, EBB
;
Anthony, AA
;
Ismail, A
;
Ismail, A
;
Lim, TS
The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. (c) 2013 Elsevier Inc. All rights reserved.