Browsing by Author "Anthony A.A."
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Publication Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: Potential application for immunoassay development(Elsevier Inc., 2013) ;Ong E.B.B. ;Anthony A.A. ;Ismail A. ;Ismail A. ;Lim T.S. ;Chancellory Building ;Universiti Sains Malaysia (USM)Universiti Sains Islam Malaysia (USIM)The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. � 2013 Elsevier Inc.