The efficiency of DNA extraction from whole blood using appropriate method is very important for molecular analysis. Therefore, the aim of this study was to compare the purity and concentration of DNA extraction method from bovine (Bos taurus), chicken (Gallus gallus), and porcine (Sus scrofa) blood. The DNA of blood samples was extracted using three types of kit, namely InnuPREP Blood DNA Mini Kit, Wizard Genomic DNA Purification Kit, and QIAamp DNA Blood Mini Kit. The results showed that blood DNA extracted using QIAamp DNA Blood Mini Kit was found to be the most effective and consistently produced high concentrated and pure DNA for three animal samples. The purity of DNA ranged from 1.73 ' 0.05 ' to 1.94 ' 0.21 ' and the range of blood DNA concentration extracted using the QIAamp DNA were between 13.73 - 2.11 and 25.01 - 2.08 ng/?l. However, the blood DNA of porcine was not successfully extracted using InnuPREP Blood DNA Mini Kit and Wizard Genomic DNA Purification Kit. These results were very crucial for the subsequent use of amplification using polymerase chain reaction (PCR) and to facilitate accurate detection in further analysis.

This study was conducted to evaluate the physicochemical characteristics and to determine the degree of consumer preference of fish burgers produced with different level of Black Tilapia surimi paste and potato flour. Five formulation of fish burgers with different percentage of Black Tilapia surimi paste to potato flour were formulated as follows: Control=70:10; A=62:18; B=66:14; C=74:6; D=78:2. The fish burgers were analysed for their colour, cooking loss, texture, pH, water holding capacity, and folding test as well as the proximate composition. Hedonic test was also carried out to evaluate the consumer preference of the fish burgers. In term of colour, raw fish burgers of formulation A was the highest (P<0.05) in the value of whiteness (L*), redness (a*), and yellowness(b*). In addition, both raw and cooked fish burger of formulation A was also significantly the highest (P<0.05) in the hardness between all formulations. However, proximate composition of raw and cooked fish burger of formulation D which contained the highest of percentage of Black Tilapia surimi paste to potato flour was significantly highest (P<0.05) in moisture and protein content. The preferenceof panellists within formulations were not significantly differed (P>0.05) in attributes of appearance, fish aroma, texture, taste, and overall acceptance except for juiciness (P<0.05). Thus, this study concluded that fish burgers produced from Black Tilapia surimi paste combining with potato flour at different level affected physicochemical properties of fish burgers but did not affect consumer acceptability.