Studies related to work ethic among santri are important studies, especially in terms of the life behavior of santri which shape the religious knowledge acquired while in Islamic boarding schools. This study shows that the Islamic work ethic among santri entrepreneurs has an important value as a medium for forming a positive mentality for business people. The Islamic work ethic also encourages the creation of competent business people in their fields. In addition, the religious knowledge possessed by Yogyakarta santri entrepreneurs has an influence in shaping the work ethic of the santri in Yogyakarta. Knowledge of santri entrepreneur training is obtained through two models, namely through teaching in Islamic boarding schools, and through interaction between santri entrepreneurs. Meanwhile, the operationalization of the Islamic work ethic in the business governance of Yogyakarta santri entrepreneurs is realized in several ways such as work arrangements, spatial planning and routine habits in the workplace