The presence of nutrients and phytochemicals including alkaloids, sterols, phenols and flavonoid in Phoenix dactylifera L. has been related to the health-benefits of date fruits consumption. Metabolomics study has been conducted to explore the human urine metabolome modifications after date fruits intake. After overnight fasting, urine samples were collected before the ingestion at 0h and every 4 hours after the consumption of date fruits at 4-24-h. Samples were analysed using LC-QTOF-MS, followed by Principal Component Analysis (PCA). Results revealed the changes of urinary metabolome during the 24 h after date fruits intake. Some phytochemicals, including alkaloids, sterols, phenols and flavonoids were appeared and disappeared after date fruits intake. These findings confirm that metabolomics is an effective tool that can be practiced in order to further discover the metabolism of phytochemicals and its relation with human health. � 2019, Indian Journal of Public Health Research and Development. All rights reserved.

Date fruits (Phoenix Dactylifera L.) are considered as an ideal food because it consists of high nutrients and provide beneficial effect to human health. Bioactive compounds in Ajwa dates have different polarities thus require different polarities of solvents for the extraction. In this study, we investigated the effects of selective extracting solvents and their combination on extraction of bioactive compounds in Ajwa flesh using mixture design. Methanol, chloroform and hexane were used in the design. Identification of bioactive compounds using GCMS shows Ajwa date flesh contain antioxidants, antifungal, anticholesterol, antimicrobial, anticancer and anti-inflammatory. Most bioactive compounds were identified in methanolic extract (D1) except ?-Sitosterol and Vitamin E were identified in hexane extract (D3). Meanwhile, Longifolenaldehyde and Nonadecyl pentafluoropropionate were identified in hexane layer (D5H) from the combination of methanol: hexane (1:1). Copyright � 2017, AIDIC Servizi S.r.l.