Browsing by Author "Fadlul Azim Fauzi Mansur"
Now showing 1 - 9 of 9
Results Per Page
Sort Options
- Some of the metrics are blocked by yourconsent settings
Publication Canine-sourced Leptospirosis(Japan Health Sciences University & Japan Internet, 2020) ;Fadlul Azim Fauzi Mansur ;Nurul Azmawati Mohamed ;Mohd Rahman OmarAbdul Aziz MarwanBackground: Leptospirosis cases are typically rodent-sourced. Domestic animals like cattle and dogs are also known to be carriers of Leptospira but are rarely implicated in human cases. Case Presentation: In this case, a parasitology researcher who dissected dogs presented with atypical leptospirosis which had high possibility to be canine-sourced. Conclusion: Leptospirosis must be suspected in febrile patients with canine bodily fluid exposure. - Some of the metrics are blocked by yourconsent settings
Publication Developing Novel Anthelmintics: The Stability Of Cysteine Proteinase Activity In A Supernatant Extract Of Papaya Latex(Cell press, 2021) ;Fadlul Azim Fauzi Mansur ;W. Luoga ;J.M. Behnke ;D.J. Buttle ;I.R. DuceM.C. GarnettPlant derived cysteine proteinases (CPs) have long been known to possess anthelmintic properties but have attracted renewed attention recently because of the acute need to discover novel methods for controlling helminth infections as a result of increasing drug resistance. However, surprisingly little is known about the stability of these proteins under typical storage and in vivo exposure conditions. We found that CPs in a supernatant preparation from papaya latex (PLS) were stable during the initial refinement process and when stored under low temperatures, but lost activity during dialysis and within 7 days of storage when kept at ambient temperature (18–20C). The enzyme activity in PLS was not affected by repeated freeze-thaw cycles and was also stable under typical in vitro assay conditions at 37C used for quantifying effects on helminths. Active enzyme activity was still detectable in the colon 3–4 h after oral administration in rodent models. - Some of the metrics are blocked by yourconsent settings
Publication Humerus Fracture Healing Using Traditional Chinese Medicine(USIM Press, 2021) ;Fadlul Azim Fauzi Mansur ;Siti Soraya Ab Rahman ;Mohd Shaffid Md. Shariff ;Mohd Asha'ari BainZetty Nadia Mohd ZainCases of mid-shaft humerus fracture are often managed conservatively. However, poor fracture healing is not uncommon and often require surgical intervention with equivocal prognosis and may result in complications such as radial nerve injury and nonunion. We report a case of successful healing of mid-shaft humerus fracture using traditional Chinese medicine (TCM). The principles of fracture management in TCM is very different from conventional orthopaedics utilizing light immobilization, active joint exercise and herbal agents that promote bone healing. This case report illustrates the potential role of traditional Chinese medicine in humerus fracture management. - Some of the metrics are blocked by yourconsent settings
Publication Improving The Embalming Protocol Under Non-ideal Conditions(Universiti Kebangsaan Malaysia (UKM), 2019) ;Mohd Azrul Asuad Mohd Zain ;Mohd Hairulhisyam NgatimanFadlul Azim Fauzi MansurPreservation of cadaver for educational purposes are central in anatomical education. The embalming process is a standardized protocol involving fixatives such as chlorine and alcohol-based preparations. Under ideal conditions of temperature and humidity, cadavers stored in storage fluid may last a long time. However, under an increase in temperature and humidity such in poorly ventilated rooms or with direct sunlight exposure, mold infestation may occur resulting in deterioration of preservation quality notable by malodorous cadaver or greenish change in storage fluid. We experimented with different combinations and volumes of storage fluid to achieve an effective protocol for cadaver preservation under non-ideal conditions of humidity and temperature. An addition of 200ml phenol to standard protocol for storage fluid preparation resulted in well preserved cadaver free from mold infestation. Phenol volume of less than 200ml or the addition of other fixatives such as chloroform in volumes exceeding the standard protocol value was not effective. Addition of 200ml phenol to storage solution standard protocol was effective against mold for cadaver preservation under non-ideal conditions. - Some of the metrics are blocked by yourconsent settings
Publication Islands of White in a Sea of Red: A Cutaneous Manifestation of Dengue Fever in a Pregnant Woman(USIM Press, 2021) ;Fadlul Azim Fauzi MansurNurul Azmawati Mohamed @ Abd AzizDengue fever is a significant public health problem in Asia, resulting in serious morbidity and even mortality. It is mainly known for causing severe constitutional symptoms and bleeding, often resulting in haemorrhagic shock. Dengue fever may manifest cutaneously in several forms. These include petechial rash, maculopapular rash and what has been termed as islands of white in a sea of red. Most can be attributed to capillary fragility, which led to tests such as the tourniquet test. This case highlights the dengue fever cutaneous presentation of islands of white in a sea of red in a multigravida woman during her first trimester. Her husband and son who was infected at the same time did not develop similar skin condition but developed the more common maculopapular and petechial rashes. The pathophysiology of such lesions is not well understood, and its relation to pregnancy is not known. Blood investigations were typical of dengue fever. The outcome of the patient’s pregnancy was also normal. This case highlights the need for doctors to look out for such lesions in dengue patients. - Some of the metrics are blocked by yourconsent settings
Publication Parasitic Worm Infection amongst the Orang Asli (aboriginal)School Children near Cyberjaya, Malaysia(Japan Health Sciences University & Japan International, 2020) ;Noor Amiza Zainal Abidin ;Faizul Helmi Addnan ;Nur Fariha Mohd Manzor ;Wan Omar Abdullah ;Noradilah Samseh Abdullah ;Mohamed Kamel AG ;Zatul-'Iffah Abu HasanFadlul Azim Fauzi MansurIntroduction: Parasitic worm infection is a global affliction with significant disease burden. The disease however is endemic in the tropics where poverty stricken communities live in substandard living conditions. In Malaysia, the infection is largely limited to Orang Asli (aborigine) and rural communities across the country where prevalence may reach up to 90%. Objective: This study investigates the parasitic worm infection in an aboriginal primary school closely located to Cyberjaya, the modern IT capital of Malaysia. Materials and Methods: 42 pupils from Bukit Cheeding primary school participated in this study and were screened for hel- minth infection by faecal sample examination using Kato-Katz technique. Result: The overall prevalence of soil-transmitted helminth (STH) infection was 59.5%. Majority of pupils were infected with Trichuris trichiura and Ascaris lumbricoides and double infection with Trichuris and Ascaris constituted 16.6% prevalence. None of the pupils were infected with hookworm. The infection rate amongst the males (66.7%) seemed to be higher than the females (46.7%). The mean worm burden for Ascaris lumbricoides was 2495.6 ± 1709 while for Trichuris trichiura was 1093.1 ± 433. Conclusion: Despite the rising affluence in Malaysia's Klang Valley, minority communities such as the aborigine who live side by side to modernity are still plagued with soil-transmitted helminth infection. More efforts are needed to address this problem. - Some of the metrics are blocked by yourconsent settings
Publication The Potential Role Of Thrombopoietin And Il-6 In The Thrombocytosis Effect Of Carica Papaya Leaves(JK Welfare & Pharmascope Foundation, 2021) ;Laila M Abdelrahim ;Zetty Nadia Mohd Zain ;Abdul Jalil SNS ;Abu Seman Z ;Fadlul Azim Fauzi MansurNoradilah Samseh AbdullahDengue fever is endemic in tropical urban developing regions worldwide. Thrombocytopenia is an important clinical feature which may result in bleeding. However, there is no specific treatment for Dengue-induced thrombocytopenia. Carica papaya leaves (CPL) is a popular remedy in South East Asia to treat Dengue-induced thrombocytopenia. Development of CPL into pharmaceutical therapeutic agents is not forthcoming due to lack of rigorous scientific evidence and unknown mechanism of action. This study investigated the role of thrombopoietin (TPO) and interleukin (IL-6) in the thrombocytosis effect of CPL in vivo. These experiments were conducted using busulfan-induced thrombocytopenic rats. Treatment of aqueous and methanol extracts of CPL at 600mg/kg were administered orally for 7 consecutive days and serum platelet count was determined intermittently until day 15. At the end of experiments, serum Thrombopoietin (TPO) and IL-6 levels were determined by ELISA. Both aqueous and methanol extracts of CPL significantly increased platelet count compared to the control groups (x2 (2) = 25.373, P = 0.00). Investigations into the mechanism of thrombocytosis showed that TPO and IL-6 levels were increased compared to controls but was not statistically significant (H (3) = 5.339 P = 0.149) (H (3) = 4.412 P = 0.220) respectively. This study is the first to document the thrombocytosis effect of both aqueous and methanol CPL extracts in a rodent model system. Our findings showed that aqueous extract of CPL demonstrated an increase of TPO and IL-6 levels. We suggested that the possible mechanism could be linked with the upregulation of major thrombopoietic cytokines such as TPO and IL-6 - Some of the metrics are blocked by yourconsent settings
Publication The Relative Indirect Anthelmintic Effect of Caprine Milk on Mucins Gene Expression in Vitro Using IL-22 Treated LS174T Cells Model of Helminth Infection(JK Welfare & Pharmascope Foundation, 2020) ;Fadlul Azim Fauzi Mansur ;Anis NA Rauf ;Nur Fariha Mohd Manzor ;Faizul Helmi Addnan ;Wan Omar AbdullahOmaima A NajmMucus secretion by intestinal goblet cells constitutes an important mechanism in TH2 response following helminth infection mediated by the key cytokine IL-22. This indirect mechanism rather than directly attacking the parasite is important in preventing helminth attachment hence promoting helminth expulsion from the intestinal tract. We hypothesized that natural products having an anthelmintic activity like caprine milk may exert similar response. Using human intestinal LS174T cells treated with IL-22 to simulate helminth infection, we tested whether or not the co-treatment with caprine milk induces MUC1, MUC3, MUC4 and MUC5B genes expression. Optimal concentrations for caprine milk was determined to be 25% and 50% from cell viability assay. IL-22 induced helminth infection model was confirmed. However, the indirect anthelmintic effect of caprine milk was only relative as treatment of caprine milk in LS174T cells and IL-22 in vitro did not significantly induce MUC1, MUC3, MUC4 and MUC5B genes expression when compared to treatment with IL-22 alone. In conclusion, caprine milk was not significantly associated with the mechanism of increased mucus production through up regulation of mucin genes by intestinal cells. Caprine milk may possess direct anthelmintic effect rather than indirect. - Some of the metrics are blocked by yourconsent settings
Publication Spleen Autotransplantation In Rat(Faz Publishing, 2020) ;Adlina Mohammad ;Affirul Chairil Ariffin ;Fadlul Azim Fauzi Mansur ;Wan Omar Abdullah ;Asral Wirda Ahmad Asnawi ;Anuar SaniRazrim RahimAim: This paper describes the surgical procedure for spleen auto transplantation in rats. Splenectomy is an important surgery protocol in research studies involving immunology and hematopoietic diseases. Spleen auto transplantation is an alternative method undertaken to preserve the splenic function as the absence of spleen (asplenia) is associated with lifelong risk for sepsis, especially by encapsulated bacteria. Method: Four six weeks old male Sprague-Dawley rats, with range of weight from 248-281 grams, underwent spleen autotransplantation via midline laparotomy incision. In this procedure the spleen was completely removed (total splenectomy); it involved removing the spleen and re-transplanted in greater omentum. A piece of the spleen weighing approximately 0.34 grams was transplanted inside the greater omentum and held in place with an absorbable suture knot. The muscle layer and skin incision were then closed using absorbable suture. The surgery was conducted under deep sedation and the rat was monitored post-operatively until it fully recovered. Outcome: The post-operative recovery of the rat was uneventful. Six-weeks after the surgery, the rat underwent a re-look laparotomy and the auto-transplanted spleen was examined. There was a three-fold increase in the size and weight of the spleen. Conclusion: This spleen auto transplantation model, which utilized an omental pouch, was not only successful in preserving the spleen, but also allowed for spleen regeneration. This model provides future platform for further research in auto-transplanted spleen.