This study focuses on identifying the best site for placement of pulse oximeter probe for accurate measuring of oxygen saturation. Twenty-three healthy male volunteers aged 20 to 40 years old were recruited in this study. Cold pressor test was done to stimulate vasoactivity and 460 measurements of SpO(2) level were obtained throughout the study. The results were analyzed using ANOVA with p < 0.05 was considered to be significant. Bias between sites are determined using Bland-Altman plot whilst the internal consistency of each measurement sites are identified via Cronbach-Alpha (alpha). Results showed that the best site for cutaneous oxygen saturation measurement is at the earlobe using the ear sensor. Portable finger pulse oximeter oxygen saturation estimation is accurate and reliable at specific fingers (thumb, right middle and right ring fingers) regardless of stimulation on peripheral vasoactivity.