‘Janus particle’ refers the generation of two particles in single system having different physical properties and two surfaces involved in the formation of Janus are having different chemistries. In this study, we created the Janus using a hybrid of Zinc Oxide (ZnO) and gold (Au) on the sensor surface towards making a high-performance DNA sensor. In the current work, we present a novel solution process which involve single-step process to synthesize Janus ZnO/Au textured film using sol-gel method.