This study aims to develop a disinfection chamber that is portable and equipped with an automatics disinfectant spray system. The automatic disinfection chamber works by implementing a mechatronics system design which involves the use of the sensor, controller, and actuator. Firstly, an ultrasonic sensor is used to detect the presence of a user at the entrance of the chamber. Then, a microcontroller that acts as a brain to the system processes the sensor reading and produces an electrical signal to activate the water pump (actuator). This allows the water pump to channel the disinfectant solution from the tank to the spray nozzle for the human disinfection procedure. The proposed disinfection chamber has several advantages including automatic operation with minimal human intervention, as well as a modular design for easy transportation and assembly.