In our previous study, we isolated eight bacterial endophytes with positive antimicrobial activity out of 82 isolates from five different places in Pulau Langkawi. The antimicrobial activity of the endophytes secondary metabolite although moderate, was further explored for the prospect to be enhanced by incorporating to silver nitrates. Only two isolates were able to synthesise silver nanoparticles namely Bacillus subtilis (B7b) and Pseudomonas resinovorans (B2c) within 72 hours of incubation at room temperature via extracellular method. Antimicrobial assay against Staphylococcus aureus, methicilin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermis, Escherichia coli and Pseudomonas aeruginosa was performed. Synthesised silver nanoparticles (AgNPs) showed excellent inhibitory effect against both Gram positive and Gram negative bacteria but secondary metabolites without AgNPs showed positive activity only against Gram positive bacteria. The results suggested that incorporation of AgNPs was able to enhance antimicrobial activity of secondary metabolites of B7b and B2c against test bacteria. � 2019, Malaysian Society of Applied Biology. All rights reserved.