Browsing by Author "Ismail, E"
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Publication Hemoglobinopathies and Thalassemia Screening among Senoi Orang Asli in Peninsular Malaysia(Amer Inst Physics, 2015) ;Rong, DKX ;Ismail, E ;Sabudin, RZAR ;Hussin, NHOthman, AOrang Asli are the minority indigenous people in Peninsular Malaysia and can be divided into 3 main groups (Negrito, Senoi and Proto Malay) with different six sub-ethnics under each group. Within the Senoi group, the six sub-ethnics are sub-ethnic Mah Meri, Semoq Beri, Che Wong, Jah Hut, Semai and Temiar. This study was aimed to investigate the current prevalence of alpha- and beta-thalassemia and hemoglobinopathies and their mutation types among all six sub-ethnics of Senoi Orang Asli. Blood samples from 685 Senoi participants were collected and sent immediately for routine full blood count analysis and hemoglobin sub-typing. Of 378 subjects screened, 7 subjects were found to be Hemoglobin E (HbE) beta thalassemia carriers, 13 beta thalassaemic, 35 Hemoglobin Constant Spring (HbCS) carriers, 6 compound HbE and HbCS carriers, 32 with HbE disease and 163 HbE heterozygote carriers. The findings of high HbE among Temiars and Jah Huts and high HbCS exclusively in Jah Huts in this study suggest distinct differences across sub-ethnics under Senoi group. Understanding of prevalence and wide spectrum of thalassemia and hemoglobinopathies among Senoi and Orang Asli is essential for national thalassaemia awareness and prevention program, especially in Orang Asli communities. - Some of the metrics are blocked by yourconsent settings
Publication Molecular Characterisation of alpha- and beta-Thalassaemia among Indigenous Senoi Orang Asli Communities in Peninsular Malaysia(Wiley, 2017) ;Koh, DXR ;Sabudin, RZAR ;Yusoff, MM ;Hussin, NH ;Ahmad, R ;Othman, AIsmail, EThalassaemia is a public health problem in Malaysia, with each ethnic group having their own common mutations. However, there is a lack on data on the prevalence and common mutations among the indigenous people. This cross-sectional study was performed to determine the common mutations of - and -thalassaemia among the subethnic groups of Senoi, the largest Orang Asli group in Peninsular Malaysia. Blood samples collected from six Senoi subethnic groups were analysed for full blood count and haemoglobin analysis (HbAn). Samples with abnormal findings were then screened for - and -globin gene mutations. Out of the 752 samples collected, 255 showed abnormal HbAn results, and 122 cases showing abnormal red cell indices with normal HbAn findings were subjected to molecular screening. DNA analysis revealed a mixture of - and -globin gene mutations with 25 concomitant cases. The types of gene abnormalities detected for -thalassaemia were termination codon (T>C) Hb CS ((CS)), Cd59 (G>A) haemoglobin Adana (Hb Adana) ((Cd59)), initiation codon (ATG>A-G) ((IniCd)), two-gene deletion (-(SEA)), and single-gene 3.7-kb deletion (-(3.7)). For -thalassaemia, there were Cd26 (G>A) Hb E ((E)), Cd19 (A>G) Haemoglobin Malay (Hb Malay) ((Cd19)), and IVS 1-5 (G>C) ((IVS 1-5)).