Browsing by Author "Ismail A."
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Publication Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: Potential application for immunoassay development(Elsevier Inc., 2013) ;Ong E.B.B. ;Anthony A.A. ;Ismail A. ;Ismail A. ;Lim T.S. ;Chancellory Building ;Universiti Sains Malaysia (USM)Universiti Sains Islam Malaysia (USIM)The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. � 2013 Elsevier Inc. - Some of the metrics are blocked by yourconsent settings
Publication Comparative study of physico-chemical analyses of different water resources in Setiu Wetland, Terengganu(Springer International Publishing, 2018) ;Lani M.N. ;Malek Rivan N.F. ;Ismail A. ;Wan Omar W.B. ;Hassan Z. ;Suhaili Z. ;Faculty of Science and Technology ;Universiti Malaysia Terengganu (UMT) ;Universiti Sains Islam Malaysia (USIM)Universiti Sultan Zainal Abidin (UniSZA)Physico-chemical properties of water are usually reflecting the functioning of an aquatic ecosystem like Setiu Wetland, Terengganu. Therefore, this study was conducted to determine the physico-chemical properties and chemical nature of their water resources (trench, rivers, drainage and tap water) and their impact on sustainable water resource in Setiu Wetland, Terengganu. A total of 12 water samples were collected aseptically from in this area and the field work was conducted from August to September 2015. In-situ measurements of physico-chemical properties of the water samples were recorded using YSI Professional Plus Parameter (XYLEM, USA) with multi-sensor probe. The analyses of physico-chemical of water include of water temperature, barometric pressure, dissolved oxygen, specific conductance, conductance, redox potential, total suspended solids, salinity and pH. In comparison to previous study conducted in 2008, most of the parameters showed that water quality at Setiu Wetland, Terengganu remained Class 1 based on National Water Quality Standards for Malaysia. In conclusion, the various water resources in Setiu Wetland was in very good condition indicating that the habitats in Setiu Wetlands have conditions suitable to support a biotic community. Further study should be carried out in monitoring water qualities of the water resources at Malaysia to increase safety of water consumption. � Springer Nature Switzerland AG 2019. - Some of the metrics are blocked by yourconsent settings
Publication Creating creative and innovative Muslim society: Bid'ah as an approach(2013) ;Zarif M.M.M. ;Nizah M.A.M. ;Ismail A. ;Mohamad A. ;Centre of Core Studies ;Islamic Science InstituteUniversiti Sains Islam Malaysia (USIM)The objectives of this preliminary work on the Islamic creativity concept are to reassess the role of bid'ah in creating creative and innovative Muslim through its conceptual and understanding framework; discover the relationship between bid'ah and the advancement of Islamic civilization; and discuss its necessity positively as highlighted in the approach of avoiding extremism or "duna ifrat wa la tafrit" in building a just and balanced Muslim society (ummatan wasatan). By employing the qualitative method through content analysis in evaluating the necessary and relevant reports, articles and texts, it is hoped that this paper will offer a distinctive perspective of bid'ah, its positive role and contribution in contemporary Muslim society. - Some of the metrics are blocked by yourconsent settings
Publication Creativity and innovation in Islam: It's necessity in Islamic education(Medwell Journals, 2015) ;Sulaiman A. ;Mohd Zarif M.M. ;Mohd Nizah M.A. ;Atoma P. ;Ismail A. ;Kandil H.M.T.E. ;Faculty of Quranic and Sunnah Studies ;Centre Core StudiesUniversiti Sains Islam Malaysia (USIM)This is a preliminary study on the Islamic concept of creative thinking through the life of Muhammad (SAW). It is aim to revisit, the concept of creativity and innovation in Islam, from the examples shown by the Prophet (SAW). This study also highlights the significance and the needs to apply the Prophetic model of Islamic creativity and innovation to the present educational system. It employed qualitative approach by theoretical and conceptual analysis of relevant historical cases to establish, the concept the Islamic creative and innovative thinking from the life of Muhammad (SAW). It is hope that it may provide a distinctive perspective on the concept of creativity and innovation and its necessity in contemporary Islamic educational system which should be treated as a crucial tool for modern era. Further actions are discussed. � Medwell Journals, 2015. - Some of the metrics are blocked by yourconsent settings
Publication Detection of butter adulteration with lard by employing 1H-NMR spectroscopy and multivariate data analysis(Japan Oil Chemists Society, 2015) ;Fadzillah N.A. ;Man Y.B.C. ;Rohman A. ;Rosman A.S. ;Ismail A. ;Mustafa S. ;Khatib A. ;Institut Pengurusan dan Penyelidikan Fatwa Sedunia (INFAD) ;Universiti Putra Malaysia (UPM) ;Universiti Teknologi Malaysia (UTM) ;Gadjah Mada University ;International Islamic University Malaysia (IIUM)Universiti Sains Islam Malaysia (USIM)The authentication of food products from the presence of non-allowed components for certain religion like lard is very important. In this study, we used proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy for the analysis of butter adulterated with lard by simultaneously quantification of all proton bearing compounds, and consequently all relevant sample classes. Since the spectra obtained were too complex to be analyzed visually by the naked eyes, the classification of spectra was carried out.The multivariate calibration of partial least square (PLS) regression was used for modelling the relationship between actual value of lard and predicted value. The model yielded a highest regression coefficient (R2) of 0.998 and the lowest root mean square error calibration (RMSEC) of 0.0091% and root mean square error prediction (RMSEP) of 0.0090, respectively. Cross validation testing evaluates the predictive power of the model. PLS model was shown as good models as the intercept of R2Y and Q2Y were 0.0853 and ?0.309, respectively. � 2015 by Japan Oil Chemists� Society. - Some of the metrics are blocked by yourconsent settings
Publication Development of clinical pathway for non-surgical management of chronic periodontitis(Malaysian Public Health Physicians Association, 2018) ;Kamil W. ;Hian O.Y. ;Mohd-Said S. ;Zainuddin S.L.A. ;Ramli H. ;Noor E. ;Ayob R. ;Aziz A.F.A. ;Ismail A. ;Sulong S. ;Mohd-Dom T.N. ;Faculty of Dentistry ;International Islamic University Malaysia (IIUM) ;Australia ;Universiti Kebangsaan Malaysia (UKM) ;Universiti Sains Malaysia (USM) ;Universiti Sains Islam Malaysia (USIM) ;Universiti Teknologi MARA (UiTM) ;Ministry of Health MalaysiaUniversiti Kebangsaan Malaysia (UKM) Medical CentreThe vast range of treatment protocols available for non-surgical management of chronic periodontitis can affect the consistency of clinical decision-making for dentists. This is further compounded by the different case definitions for periodontitis used in various clinical studies. The aim of this paper is to describe the steps taken leading to an expert consensus of periodontitis case definition followed by the development of a clinical pathway for managing chronic periodontitis. To assist reaching a consensus on a standard case definition of periodontitis for clinical research, a roundtable discussion was held involving 13 dental specialists and researchers from universities and the Ministry of Health. Participants discussed clinical experiences in identifying periodontitis and related issues based on scientific evidence. A further expert panel discussion consisting of 8 periodontists was conducted at another session to review current management practices and evidence-based practices available from the literature and generated a clinical pathway for non-surgical management of periodontitis. The case definition derived from the roundtable discussion recognises differentiation of selected clinical parameters and their thresholds as well as the extent and severity of the periodontitis. As for the newly developed clinical pathway, experts collectively defined the appropriate goals to satisfy the multidimensional needs of the patients which are translated into detailed elements of care, including the sequence of events of patients at each dental visit over time. The agreed case definition will facilitate selection and recruitment of cases for clinical studies while the clinical pathway can be used to reduce variations between clinicians. � 2018, Malaysian Public Health Physicians Association. - Some of the metrics are blocked by yourconsent settings
Publication Entrepreneurs’ characteristics from the islamic perspective: A study of muslimpreneurs’ performance(Hampstead Psychological Associates, 2020) ;Ismail A. ;Ismail F.L.M. ;Wahab N.A. ;Latiff L.A. ;Kandil H.M.T.E. ;Centre of Core Studies ;Faculty of Economics and MuamalatUniversiti Sains Islam Malaysia (USIM)This research aims at identifying the Islamic characteristic factors of Muslimpreneures in the business environment. The main sources of this study involve the adaptation characertistics of Muslim scholars in a business environment, including Quran, Hadith, narrations, academic study as well as other reliable sources. As such, this research is an attempt to explore the factors of Islamic characteristic elements of Muslimpreneurs, such as taqwa and planning, and it is hoped to contribute to the further development and understanding of this area in the humanities and social science fields. Moreover, the study will look at the objectives of Muslim entrepreneurs in Islam as part of fulfilling the duty of a vicegerent on earth, strengthening one�s relationship with Allah, finding truth and serving the society. The findings revealed that significant relationships exist between the state of taqwa and planning among Muslimpreneurs in relation to business performance. These findings provide a distinctive perspective on the concept of taqwa and proper planning and its necessity in the Islamic system for the contemporary modern era. In addition, it recommends to investigate the trend and profile of successful Muslimpreneurs and eventually develop global Muslimpreneurs. The outcomes and results of this study are then hoped to stimulate further improvements, advancements and research on the subject matter. � 2020, Hampstead Psychological Associates. All rights reserved. - Some of the metrics are blocked by yourconsent settings
Publication The identification and detection of a unique gene in Salmonella enterica subsp. enterica Weltevreden using PCR-based approach(2014) ;Tan C. ;Yuan Xin G. ;Chiann Ying Y. ;Ismail A. ;Kia Kien P. ;Islamic Science Institute ;Universiti Sains Malaysia (USM)Universiti Sains Islam Malaysia (USIM)Introduction: Salmonella enterica are important food-borne pathogens that cause human gastroenteritis, bacteremia and subsequent focal infections. The incidences of food-borne salmonellosis due to non-typhoidal Salmonella serotypes are increasing periodically in Malaysia. Other than the two predominant non-typhoidal Salmonella serotypes, S. Typhimurium and S. Enteritidis, an emerging serotype, S. Weltevreden, is increasingly being isolated from hospitalized-gastroenteritis patients in Malaysia. Serotyping is the conventional method for differentiation of this pathogen from the 2500 other serotypes known to exist. However, this method has a number of disadvantages including low specificity and high cost. Therefore, a molecular method for identification of S. Weltevreden in clinical isolates based on amplification of a specific DNA sequence present in its genome is proposed. Methods: A total of 234 clinical isolates were collected from the Department of Clinical Microbiology and Parasitology, HUSM from year 2010-2012. There were 49 S. Weltevreden isolates which have been confirmed by the Institute of Medical Research (IMR) using conventional serotyping method. Two sets of primers targeting a unique gene in S. Weltevreden (SWE349) and a pan- Salmonella gene (invA284) were designed using Primer3 online software, and a multiplex-PCR (mPCR) assay was developed to detect S. Weltevreden in clinical isolates. The optimized S. Weltevreden mPCR assay was evaluated on a panel of known Salmonella and non- Salmonella spp. to determine the specificity and sensitivity of the mPCR assay. Results & Discussion: The S. Weltevreden serovar specific primer, SWE349, and the internal control primer, invA284, resulted in 2 PCR products of 349bp and 284bp, respectively. The mPCR assay for S. Weltevreden targeting gene SWE349 showed 100% sensitivity (49/49) and specificity (36/36) in concordance with the conventional serotyping method in detecting only S. Weltevreden clinical strains, but not from the panel of 26 non-typhoidal Salmonella and 10 non- Salmonella isolates. Conclusion: A cost effective, sensitive and specific mPCR assay has been developed for the detection and confirmation of Salmonella Weltevreden from clinical isolates of gastroenteritis patients, using genes SWE349 and invA284. � 2014 Asian Pacific Tropical Medicine Press. - Some of the metrics are blocked by yourconsent settings
Publication Phage display antibodies for diagnostic applications(2013) ;Hairul Bahara N.H. ;Tye G.J. ;Choong Y.S. ;Ong E.B.B. ;Ismail A. ;Lim T.S. ;Universiti Sains Malaysia (USM)Universiti Sains Islam Malaysia (USIM)With major developments in molecular biology, numerous display technologies have been successfully introduced for recombinant antibody production. Even so, phage display still remains the gold standard for recombinant antibody production. Its success is mainly attributed to the robust nature of phage particles allowing for automation and adaptation to modifications. The generation of monospecific binders provides a vital tool for diagnostics at a lower cost and higher efficiency. The flexibility to modify recombinant antibodies allows great applicability to various platforms for use. This review presents phage display technology, application and modifications of recombinant antibodies for diagnostics. � 2013 The International Alliance for Biological Standardization. - Some of the metrics are blocked by yourconsent settings
Publication A preliminary study on the Islamic creativity practices(Mediterranean Center of Social and Educational Research, 2015) ;Nizah M.A.M. ;Ismail A. ;Zarif M.M.M. ;Mohamad A.S. ;Kandil H.M.T.E. ;Atoma P. ;Centre of Core StudiesUniversiti Sains Islam Malaysia (USIM)Creativity is the natural ability to generate new ideas and/or possibilities in solving problems. It is an important skill that should be practiced by students in higher education and can be nurtured by the use of various learning methods and techniques. This preliminary study conducted aims to measure the level of creativity practices among 30 volunteers of first-years student in Universiti Sains Islam Malaysia. The test instrument administered based on the Torrance Tests of Creative Thinking to the selected respondents to measure the level of creativity, creativity practices and creativity from the Islamic perspective. Data then analysed using descriptive through mean analysis and inferential statistical methods. The results showed that although there is no significant difference in the level and creativity practices among gender, but in terms of Islamic creativity elements, significant difference is observed. This indicates that the respondents are more creative upon exposure to materials on creativity during the period of the study. Thus, relevant efforts must be concentrated to produce more creative students who also have a proper understanding of creativity from the Islamic perspective. � 2015, Mediterranean Center of Social and Educational Research. All rights reserved. - Some of the metrics are blocked by yourconsent settings
Publication Salmonella Typhi TolC protein: A molecular dynamics investigation(2014) ;Leong W. ;Lim T.S. ;Ismail A. ;Choong Y.S. ;Islamic Science Institute ;Universiti Sains Malaysia (USM)Universiti Sains Islam Malaysia (USIM)Introduction: The TolC family of proteins of Escherichia coli and other pathogenic Gram negative bacteria plays an important role as an outer membrane channel for the expulsion of drug and toxin from the cell. Typhoid fever, caused by Salmonella enterica serovar Typhi kills more than 200 000 lives annually. In S. Typhi, a TolC outer membrane protein was found to be antigenic for this pathogen. Understanding the dynamics of the S. Typhi TolC protein is essential towards development of diagnostics, therapeutics and vaccine towards typhoid fever. Therefore, molecular dynamics simulation has been performed on the protein. Objective: To refine homology structure of S. Typhi TolC protein by molecular dynamics simulation. Methods: A homology model of the S. Typhi TolC protein was embedded in phosphatidylethanolamine lipid bilayer. Water molecules were added and the system was subsequently neutralized. Minimization was performed by steepest descent method following which; temperature was equilibrated at 310.15K in the NVT ensemble. A 1 ns NPT run was performed with restraint on protein atoms to maintain pressure at 1 bar. Finally, production simulation was performed for 20 ns without restraints in the NPT ensemble. The average structure of the protein was generated by RMS fitting of all protein atoms from the production run and averaging over the coordinates. Results & Discussion: Results showed that the membrane simulation was energetically stable and reproduced experimental value. The protein conformation was also stable in simulation. Conformational analysis showed that the protein is highly mobile at the extracellular loops and periplasmic helices region. From Ramachandran plot and Errat analysis, the average structure showed improvements over the initial model. Conclusion: The average structure could be advantageous in aiding design efforts for inhibitors and binders against the S. Typhi TolC protein. � 2014 Asian Pacific Tropical Medicine Press. - Some of the metrics are blocked by yourconsent settings
Publication Temperature-regulated expression of outer membrane proteins in Shigella flexneri(2013) ;Harikrishnan H. ;Ismail A. ;Banga Singh K.-K. ;Islamic Science Institute ;Universiti Sains Malaysia (USM)Universiti Sains Islam Malaysia (USIM)Background: Bacteria exist widely in a diversity of natural environments. In order to survive adverse conditions such as nutrient depletion, biochemical and biological disturbances, and high temperature, bacteria have developed a wide variety of coping mechanisms. Temperature is one of the most important factors that can enhance the expression of microbial proteins. This study was conducted to investigate how outer membrane proteins (OMPs) of the bacterium Shigella flexneri respond to stress, especially during fever when the host's body temperature is elevated. Methods. OMPs of S. flexneri ATCC 12022 and clinical isolate SH057 were extracted from an overnight culture grown at 37, 38.5, and 40�C. Comparisons of the expressed proteins under the different growth conditions were based on equal numbers of bacterial cells loaded in the SDS-PAGE gels. Separated proteins were stained with Coomassie brilliant blue. Selected proteins showing increased expression at 38.5 and 40�C were characterized by performing MALDI-ToF-ToF. Results: Different degrees of expression were demonstrated for different proteins expressed at 37�C compared to 38.5 and 40�C. The proteins with molecular sizes of 18.4, 25.6, and 57.0 kDa showed increased expression level at increasing temperature and were identified as Dps, WrbA, and PepA, respectively. Conclusion: This study revealed that strains of S. flexneri respond at the proteomic level during stress caused by elevated temperature by decreasing the expression of proteins, maintaining the level of important proteins, or enhancing the levels of proteins presumably involved in survival and virulence. � 2013 Harikrishnan et al.; licensee BioMed Central Ltd.