The Chromolaena odorata is often referred to as the Siam Weed and is a member of the Asteraceae family. Known locally as the Kapal Terbang plant, it is noted for its various health benefits given that several parts of this herb have been used to treat wounds, burns, and skin infections in addition to possessing qualities namely anti-inflammatory, antimicrobial, and antioxidant properties. Hence, the purpose of conducting this research is to contribute more information on the DNA extraction of Chromolaena odorata. Subsequent DNA extraction protocols will hinge upon the application of a commercial kit from Promega. The quantification of extracted DNA will be meticulously assessed against a Lambda DNA/ HindIII Marker standard, employing electrophoresis within a 1% agarose gel in the research findings. This systematic approach holds promise for shedding light on the genetic underpinnings of the plant's medicinal properties, potentially paving the way for novel applications in the field of healthcare and medicine. In addition, it endeavours to provide scientific evidence to support the identification of Chromolaena Odorata