Trichoderma reesei sp is an anamorph fungi which involves asexual reproduction to form discrete anamorphs in mold like configuration. It also has high rate of mycelium growth of 100% and rate of methylene blue biodegradation of 100% by day 6 in culture medium. Since methylene blue dye is one of the dyes involved in dye pollution, fungi treatment is found as most immaculate compared to other technologies in our current timeline. Agar medium is first-handedly prepared in the laboratory which consists of nutrient agar (20g/L) and glucose (20g/L). At a subsequent time, second culture of agar medium is effectuated by adding methylene blue dye (50mg/L) in agar medium to calculate the rate of methylene blue dye decolourisation before proceeding with the physico-chemical parameters determination in terms of carbon source and nitrogen source for optimum growth in a conducive environment, ascertaining the rate of methylene blue decolourisation. Liquid medium is prepared prior to the investigation of the physico-chemical parameters. By keeping pH 8 and temperature of 37°c constant, the optimum carbon source is determined by contrasting the carbon source concentration (5g/100mL, 10g/100mL, 15g/100mL, 20g/100mL, 25g/100mL, 30g/100mL) corresponding to nitrogen source determination by also contrasting the nitrogen source concentration. Methylene blue decolourisation in optimum conditions is then analysed using UV-vis spectrophotometry.

Authenticity is an important issue nowadays as humans are concerned about the quality of food they consume.Goat's milk contains more protein. carbohydrate, vitamins and minerals compare to other milk such as cows's milk. It contains less lactose and smaller fat globules than cow's milk. The composition of the goat's milk is known by people nowadays, especially the one who has lactose intolerant who cannot consume lactose and who are looking for a better milk nutrient. In this regard, determining the origin of milks using their deoxyribonucleic acid (DNA) composition is of high importance. For this research, we will determine the authentication of commercialized also known as factory goat's milk by comparing it with fresh also known as raw farm goat's milk using DNA extraction method with the PROMEGA Kit of Genomic DNA. Thus, it can show the presence of milk origin by DNA in each of the milk samples collected. This study shows clear presence of DNA of milk and serve as a parameter for quality control of milk.