Browsing by Author "Liyana Amalina Binti Adnan"
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Publication Biodegradation of Bis-Azo Dye Reactive Black 5 byWhite-Rot Fungus Trametes gibbosa sp. WRF 3 and Its Metabolite Characterization(Springer International Publishing Switzerland, 2014) ;Liyana Amalina Binti Adnan ;Abdull Rahim Mohd Yusoff ;Tony HadibarataAmeer Badr KhudhairThe culture of Trametes gibbosa sp. white-rot fungi (WRF) 3 under mesophilic conditions can lead to the degradation of azo dye compounds. This ability of T. gibbosa sp. WRF 3 is attributed to the released enzymes that are able to catalyze the structural degradation of the azo dye compound. The effect of environmental factors such as carbon sources, nitrogen sources, and pH of growth medium were investigated in this research. The addition of 20 g/L glucose (carbon source) and yeast extract (nitrogen source) at pH 5 of growth medium enhanced the decolorization of Reactive Black 5 (RB5) dye up to 87.07 % within 30 days of incubation. The decolorization of RB5 can be analyzed using UV-vis spectroscopy and differential pulse cathodic stripping voltammetry (DPCSV). The maximum absorbance of RB5 was at 597 nm and decreased after the dye was treated with T. gibbosa sp. WRF 3. In the voltammetric analysis, we examined the effect of pH of Britton-Robinson buffer (BRB) medium on the detection of bis-azo compound of RB5. A stock solution of RB5 was used in the study, and it showed two reduction peak potentials at ?0.5 and ?0.7 V which attributed to the bis-azo bond, whereas the metabolic product showed one reduction peak at ?0.6 V. The GC-MS mass spectrum confirmed the formation of metabolites at tR 4.63 min and m/z of 73 after 30 days of incubation which was sec-butylamine. - Some of the metrics are blocked by yourconsent settings
Publication Functional group determination of Neptunia Oleracea known as Malaysian water mimosa (pucuk setangki) using Fourier-Transform Infrared(Kolej PERMATA Insan, 2023-12-23) ;Muhammad Faidhi Bin Muhammed Ali ;Muhammad Haziq Fikri Bin Hanafee ;Izz Awwad Bin Muhd Riza ;Ahmad Hakimi Bin Shaffie ;Liyana Amalina Binti Adnan ;Rahayu Binti AhmadTg Ainul Farha Binti Tg Abdul RahmanNeptunia Oleracea is locally known as Pucuk Setangki (Water Mimosa) that belongs to the Fabaceae family which is a herbal plant that is believed originated from India, Southeastern Asia and Tropical Africa. It is commonly known for its benefits as first aid medicine and food ingredients. Usually, Water Mimosa is used in the traditional medicines of Malaysia. It is part of treatments for earaches and even syphilis. It is also used as food ingredients such as masak lemak cili api (cook chili fat). Nowadays, people tend to use modern medicine that they have forgotten about the traditional herbs that our ancestors used. Thus, the goal of this research is to analyze the functional group of Neptunia Oleracea extract. Specifically, the research will focus on the functional group that is in the pucuk setangki. It can also increase people's knowledge of pucuk setangki and encourage them to use it more often. In order to prove its efficacy, functional group analysis of Neptunia Oleracea leaf extract was performed using Fourier Transform Infrared (FTIR). The spectrum and wavelength obtained can be used to identify the functional group and thus, the antimicrobial compound was discovered present in the plant. Based on this study, Neptunia Oleracea leaves do have antimicrobial compounds. Specifically, the research will focus on the functional group in the pucuk setangki. This research reminds people of the importance of pucuk setangki and its properties. This research is a contribution for other researchers to make other products based on this research and to help others to realize the benefits in pucuk setangki. The result will be shared throughout people so it can be use by people in the future.