Abstract: The purpose of this research is to assess the formation and determination of cholesterol oxidationproducts (COPs) in meat and poultry products and its effects on human health. Cholesterol is a molecule withan unsaturated or double bond which is prone towards oxidation and formation of COPs. The impact of COPstowards human health depend on the type of COPs formed. Extraction, purification and detection methods inquantifying COPs play an important role in the analysis as COPs occur mostly at low levels. Direct coldsaponification was the most suggested method due to high efficiency, good method precision, minimal artifactformation and ease of handling to recover COPs. High performance liquid chromatography (HPLC) and gaschromatography (GC) couple with mass spectrometry (MS) detector have become more important in providingbetter quantitative information in COPs analysis. Determination of cholesterol using gas chromatographyusually need to go through derivatisation process to improve volatility and thermo stability, to optimize peakshape, to decrease retention time and to increase sensitivity. However, it makes no difference between GCanalysis of free cholesterol and those of trimethylsilyl (TMS) or acetate derivatives. The gas chromatographymass spectrometry (GCMS) is the most commonly used method to determine COPs for many food samplesincluding meats and poultry. Key words: Cholesterol Oxidation Products Cholesterol Gas Chromatography Meat Poultry

Abstract: Cholesterol Oxidation Products (COPS) are well known for their negative biological effects. Till now,there is no study reported on solvent extraction used for COPs. In this study, two solvent extractiontechniques, namely chloroform/methanol (2:1, v/v) or Folch method and n-hexane/2-propanol (3:2, v/v) orHara-Radin method of cholesterol and COPs in beef tallow and lard were compared. Cholesterol and COPs(5-cholestane, 7-ketocholesterol and 25-hydroxycholesterol) contents in both fats also analyzed. The analysispassed through four major steps; extraction of lipids, saponification, enrichment of COPs and quantificationby gas chromatography mass spectrometry triple quadrupole. All standards showed good linearity withcorrelation coefficient (r ) of 0.9998 (5-cholestane), 0.9999 (cholesterol), 0.9873 (25-hydroxycholesterol) and20.9693 (7-ketocholesterol). Data obtained by this method was analyzed based on precision and recovery criteria.Precision measured as standard deviation (SD) was between 0.0040 and 2.2460; and no significant different(P>0.05) for the recovery testing using 5 -cholestane in both method. Recovery by Hara Radin method in lard(27.23%) and beef tallow (8.73%) was higher than the Folch method, 14.25% and 2.97% respectively. This studyimplies that Hara-Radin method can be an alternative method to avoid the use of harmful solvent such aschloroform in Folch method. Key words:Cholesterol Oxidation Products Gas Chromatography Mass Spectrometer Triple Quadrupole Fat Solvent Extraction