Preeclampsia is a pregnancy-specific disorder characterized by the presence of hypertension with the onset of either proteinuria, maternal organ or uteroplacental dysfunction. Preeclampsia is one of the leading causes of maternal and fetal mortality and morbidity worldwide. However, the etiopathologies of preeclampsia are not fully understood. Many studies have indicated that genes are differentially expressed between normal and in the disease state. Hence, this study systematically searched the literature on human gene expression that was differentially expressed in preeclampsia. An electronic search was performed through 2019 through PubMed, Scopus, Ovid-Medline, and Gene Expression Omnibus where the following MeSH (Medical Subject Heading) terms were used and they had been specified as the primary focus of the articles: Gene, placenta, preeclampsia, and pregnancy in the title or abstract. We also found additional MeSH terms through Cochrane Library: Transcript, sequencing, and profiling. From 687 studies retrieved from the search, only original publications that had performed high throughput sequencing of human placental tissues that reported on differentially expressed genes in pregnancies with preeclampsia were included. Two reviewers independently scrutinized the titles and abstracts before examining the eligibility of studies that met the inclusion criteria. For each study, study design, sample size, sampling type, and method for gene analysis and gene were identified. The genes listed were further analyzed with the DAVID, STRING and Cytoscape MCODE. Three original research articles involving preeclampsia comprising the datasets in gene expression were included. By combining three studies together, 250 differentially expressed genes were produced at a significance setting of p < 0.05. We identified candidate genes: LEP, NRIP1, SASH1, and ZADHHC8P1. Through GO analysis, we found extracellular matrix organization as the highly significant enriched ontology in a group of upregulated genes and immune process in downregulated genes. Studies on a genetic level have the potential to provide new insights into the regulation and to widen the basis for identification of changes in the mechanism of preeclampsia. Integrated bioinformatics could identify differentially expressed genes which could be candidate genes and potential pathways in preeclampsia that may improve our understanding of the cause and underlying molecular mechanisms that could be used as potential biomarkers for risk stratification and treatment. � 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Parasitic helminth infection is a global affliction affecting both of people and livestock causing significant economic loss. Control however has not been successful due to political neglect and the development of resistance to conventional chemotherapy. Novel alternative compounds are urgently needed which resulted in screening of largely plant based sources for anthelmintic property. Animals are also important sources for medicine. Milk which has been mentioned in holy texts is a wholesome food essential for growth and health. It has also been shown to promote health due to its balanced nutritive profile and presence of bioactive components. A systematic review was performed to investigate the anthelmintic effect of milk using five scientific databases; EBSCOhost, PUBMED, EMBASE, OVID, and SCOPUS. The search included any studies related to anthelmintic effects of milk specifically animal-based milk but not those related to milk production or plant-based milk. The search yielded 353 manuscripts but only 8 manuscripts were taken into consideration after reviewing their title, abstract and full paper using pre-determined inclusion and exclusion criteria. Five articles demonstrated the anthelmintic effects of bovine milk in vitro and in vivo. One article reported antiparasitic effect in breast fed babies. One article demonstrated the superior anthelmintic effects of camel milk in vitro while cow, ewe and goat milk exhibited weak ovicidal only effects. In conclusion our systematic review yielded limited anecdotal evidence of anthelmintic property of milk. Bovine, human and camel milk showed good potential for future use in helminth control. However, the exact mechanism by which milk exhibited anthelmintic effect remains unclear. � 2017 American Scientific Publishers All rights reserved.