Purpose: This study aimed to evaluate the protective effects of edible bird nest (EBN)against the detrimental impact of Wi-Fi on male reproductive health. Specifically, itexamines whether EBN can mitigate Wi-Fi-induced changes in male reproductive hor-mones, estrogen receptors (ER), spermatogenesis, and sperm parameters.Methods: Thirty-six adult male rats were divided into six groups (n = 6): Control,Control EBN, Control E2, Wi-Fi, Wi-Fi+EBN, and Wi-Fi+E2. Control EBN and Wi-Fi+EBN groups received 250 mg/kg/day EBN, while Control E2 and Wi-Fi+E2 groupsreceived 12 μg/kg/day E2 for 10 days. Wi-Fi exposure and EBN supplementationlasted eight weeks. Assessments included organ weight, hormone levels (FSH, LH,testosterone, and E2), ERα/ERβ mRNA and protein expression, spermatogenic mark-ers (c-KIT and SCF), and sperm quality.Results: Wi-Fi exposure led to decreased FSH, testosterone, ERα mRNA, and spermquality (concentration, motility, and viability). EBN supplementation restored serumFSH and testosterone levels, increased serum LH levels, and the testosterone/E2ratio, and normalized mRNA ERα expression. Additionally, EBN increased sperm con-centration in Wi-Fi-exposed rats without affecting motility or viability.Conclusions: EBN plays a crucial role in regulating male reproductive hormones andspermatogenesis, leading to improved sperm concentration. This could notably ben-efit men experiencing oligospermia due to excessive Wi-Fi exposure