Browsing by Author "Mohd Firdaus-Raih"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
- Some of the metrics are blocked by yourconsent settings
Publication The Glaciozyma Antarctica Genome Reveals An Array Of Systems That Provide Sustained Responses Towards Temperature Variations In A Persistently Cold Habitat(Public Library of Science (PLOS), 2018) ;Mohd Firdaus-Raih ;Noor Haza Fazlin Hashim ;Izwan Bharudin ;Mohd Faizal Abu Bakar ;Kie Kyon Huang ;Halimah Alias ;Bernard K. B. Lee ;Mohd Noor Mat Isa ;Shuhaila Mat-Sharani ;Suhaila Sulaiman ;Lih Jinq Tay ;Radziah Zolkefli ;Yusuf Muhammad Noor ;Douglas Sie Nguong Law ;Siti Hamidah Abdul Rahman ;Rosli Md-Illias ;Farah Diba Abu Bakar ;Nazalan Najimudin ;Abdul Munir Abdul MuradNor Muhammad MahadiExtremely low temperatures present various challenges to life that include ice formation and effects on metabolic capacity. Psyhcrophilic microorganisms typically have an array of mechanisms to enable survival in cold temperatures. In this study, we sequenced and analysed the genome of a psychrophilic yeast isolated in the Antarctic region, Glaciozyma antarctica. The genome annotation identified 7857 protein coding sequences. From the genome sequence analysis we were able to identify genes that encoded for proteins known to be associated with cold survival, in addition to annotating genes that are unique to G. antarctica. For genes that are known to be involved in cold adaptation such as anti-freeze proteins (AFPs), our gene expression analysis revealed that they were differentially transcribed over time and in response to different temperatures. This indicated the presence of an array of adaptation systems that can respond to a changing but persistent cold environment. We were also able to validate the activity of all the AFPs annotated where the recombinant AFPs demonstrated anti-freeze capacity. This work is an important foundation for further collective exploration into psychrophilic microbiology where among other potential, the genes unique to this species may represent a pool of novel mechanisms for cold survival. - Some of the metrics are blocked by yourconsent settings
Publication Identification Of sRNA Mediated Responses To Nutrient Depletion In Burkholderia Pseudomallei(Nature Research, 2017) ;Hirzahida Mohd-Padil ;Nadzirah Damiri ;Suhaila Sulaiman ;Shiao-Fei Chai ;Sheila NathanMohd Firdaus-RaihThe Burkholderia genus includes many species that are known to survive in diverse environmental conditions including low nutrient environments. One species, Burkholderia pseudomallei is a versatile pathogen that can survive in a wide range of hosts and environmental conditions. In this study, we investigated how a nutrient depleted growth environment evokes sRNA mediated responses by B. pseudomallei. Computationally predicted B. pseudomallei D286 sRNAs were mapped to RNA-sequencing data for cultures grown under two conditions: (1) BHIB as a nutrient rich media reference environment and (2) M9 media as a nutrient depleted stress environment. The sRNAs were further selected to identify potentially cis-encoded systems by investigating their possible interactions with their flanking genes. The mappings of predicted sRNA genes and interactions analysis to their flanking genes identified 12 sRNA candidates that may possibly have cis-acting regulatory roles that are associated to a nutrient depleted growth environment. Our approach can be used for identifying novel sRNA genes and their possible role as cis-mediated regulatory systems.