The focus on the collection of prayers (du’a) contained in the hadith of the Prophet SAW has revealed the greatness and benefits behind them. A detailed observation in the content of the prayers will be able to reveal various important matters that the Prophet emphasized, whether they are in the form of a request, or in the form of restraint. Thus, prayers of the Prophet SAW can be depicted as the Prophet's guidance for Muslims in facing the journey of life in this world and in the hereafter. Therefore, this study serves to thematically analyze the collection of hadith prayers of the Prophet SAW through detailed observation of the hadiths of prayers in al-Kutub al-Sittah. The results of the study concluded that the supplications of the Prophet SAW are comprehensive and cover all aspects and conditions of daily human lives. Besides that, this study also manifest various important themes contained in Prophet SAW's prayers that always can be used as guidance by all Muslims as a way to face the tribulations in this world and in the afterlife.