Conventional types of fertilizer such as chemical and synthetic fertilizers have demonstrated various adverse effects on the environment, crops, and humans. The utilization of plant probiotics as a bio-fertilizer in agriculture has been recognized to benefit the growth of the plant and inhibit the activity of plant pathogens. Traditional formulations of bio-fertilizer have provided insight into the beneficial use of microorganisms in crops. Despite its advantage to the environment, the effectiveness of traditional bio-fertilizer is common as compared to chemical fertilizer. Thus, a variety of bio-fertilizer formulations have been developed to improve the success rate of bio-fertilizer in increasing plant productivity. This review was focused on the development of bio-fertilizer formulation and the potential of bio-fertilizer to substitute chemical fertilizer application. In addition, this research review was also undertaken with a great demand on producing low cost and highly effective fertilizer without harming the environment and humans. Thus, the advantages and disadvantages of each formulation type have also been reviewed, emphasizing the perspective of bio-fertilizer and their suitability as bio-fertilizer as a substitute for chemical fertilizers in sustainable agriculture.

Duckweed species are nutritionally meaningless plant which grow wildly in unattended areas. Therefore, understanding the metabolites content in duckweed species is essential for designing a future food products. Here, we report an untargeted Gas Chromatography-Mass Spectrometry (GC-MS)-based metabolomics approach for comprehensively discriminating between Lemna minor and Wolffia globosa of duckweeds species using principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). Ten differential metabolites levels were tentatively identified between L. minor and W. globosa. Relative to W. globosa, L. minor appeared to enrich with 5-Hydroxyl-L-tryptophan, Tocopheryl acetate, Naringenin, α-linolenic acid and glutamic acid. Furthermore, the nutritional and microbial analyses of ice cream formulated with dried L. minor were investigated. The nutritional analysis results show that relative to control, the ice cream with 2% dried L. minor had significantly increased protein, fiber and ash content. In addition, total plate count (TPC) for microbial analysis of duckweed ice cream was performed. The result suggested that the small amount of bacteria (3.82 cfu/g) was traced in formulated ice cream with 2% dried L. minor. Overall, the metabolites profile, nutritional and microbial analyses of food used L. minor plant indicate that duckweed is a good candidate for future food.

Animal slaughtering is the most vital step in the preparation of chicken meat. The step ensures the meat is safe for human consumption since the procedure of animal slaughtering can affect the meat quality. In this study, we compare the quality of chicken meats obtained via two common slaughtering procedures: neck slaughtering (NS) and neck pocking (NP). The meat quality was assessed based on physicochemical analyses (ultimate pH, colour, heme iron content, drip and thaw loss measurements and TBARS value) and metabolite profiling (FTIR, GC–MS and UHPLC with PCA and PLS-DA). The study found that, relative to the NP-, the NS-chicken meat had slightly acidic pH, and much lower values of the following parameters: L* (lightness) (P = 0.023), heme iron content (P < 0) and TBARS (P < 0.01). Comparing FTIR spectra, the metabolite fingerprints of both meats looked slightly different. This was confirmed to be due to a set of differential metabolites present in the NS and the NP-chicken meats, as recorded by GC–MS and UHPLC-TOF-MS data after analyzing with PCA and PLS-DA. Compared to the NP, the NS-chicken meat was rich in metabolites with health benefits, including n-3-polyunstaurated fatty acids (PUFA), triglyceride (TG), cytadine and uridine. In addition, the NS-chicken meat also contained much lower concentrations of free amino acids. This is desirable as free amino acid-deficiency is able to suppress the production of biogenic compounds, a group of chemicals that produce toxicological effects on human heath when taken excessively.

The soap-making process involves heating, encompassing various mechanisms such as oxidation and thermal reactions. These reactions can potentially modify the chemical structure of both animal and vegetable oils, making it challenging to trace their original sources in processed products. In this study, seven distinct bar soaps were produced using the saponification method, employing locally available commercial oils, including Canola Oil (LA), Coconut Oil (CN),Corn Oil (CO), Olive Oil (OV), Palm Oil (PO), Sunflower Oil (SF), and animal fat from Lard (LD). The chemical profiles of the raw lard and vegetable oils, as well as their respective soaps and the fats/oils extracted from the soap, were obtained using Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy, followed by analysis through a chemometric approach. Principal Components Analysis (PCA) was employed after pretreatment of the FTIR dataset to discern their distribution in a score plot for determining clustering. For clustering lard from vegetable oils, the wavenumbers ranging from 1500 to 1000 cm-1 were selected in the PCA analysis. The extraction of fats/oils from the final product, coupled with the chemometric method, successfully demonstrated the differentiation of soaps produced from vegetable oils and animal fats commonly sold in the market.

The book of Tib MSS2515 is considered a complete manuscript discussing medicine in the Malay world. The study aims to assess the use of traditional medicine and its treatment of skin diseases [focuses on shingles, leprosy, and abscess] in the MSS2515 manuscripts. Textual studies and data extraction are applied using philological methods in identifying diseases and treatment and their categorisation. The pharmacological aspect of [materia medica] is analysed by comparing scientific reports in Google Scholar and PubMed and matching the ingredients with the purpose. Findings showed there are 35 formulations for three types of skin diseases. Part of the pharmacology and its use in the manuscripts is supported with contemporary scientific evidence. Scientific studies should be intensified to confirm the safety and efficacy of formulations in Malay medical manuscripts, as their content has the potential to generate new drugs. This research also contributes to the preservation of Malay medical culture and heritage.

he mangrove ecosystem contains sediment microorganisms that play a crucial part in the decomposition of organic matter and the cycling of water and nutrients in the mangrove. Here we present the metagenomics whole genome shotgun (mWGS) sequence data analysis from three soil samples that were collected at the freshwater riverine mangrove at Lukut River, Negeri Sembilan, Malaysia. Data analysis shows different distributions of bacteria of the genera Bradyrhizobium, Methyloceanibacter and Desulfobacteaceae were detected in soil samples collected at freshwater riverine mangrove. In the data analysis, we report the existence of a large number of Carbohydrate-Active genes in metagenomes collected from mangrove soil. An in-depth exploration of functional annotation analysis based on the KEGG database also showed that the most abundant genes found in these three soils are those that function in carbon fixation pathways, followed by methane, nitrogen, sulfur metabolisms, atrazine and dioxin degradations

Duckweed is a future food and a source of affordable protein that has the potential to replace animal protein. This study aims to formulate a bio-fertilizer consisting of mangrove-associated bacteria to boost the growth and protein of duckweeds as a sustainable approach to increase plant-based protein yields. The culture-depending technique was performed by using Aleksandrow agar, Pikovskaya’s agar, and Jensen agar to screen potassium-solubilizing bacteria, phosphate-solubilizing bacteria and nitrogen-fixing bacteria, respectively, from mangrove soil sediments. Mangrove-associated bacteria that are close to Acinetobacter radioresistens, Brachybacterium paraconglomeratum, and Enterobacter cloacae, which are known as nitrogen-fixing bacteria, Klebsiella quasipneumoniae, Bacillus tropicus, and Paenibacillus pasadenensis known as potassium-solubilizing bacteria, and Bacillus cereus and Bacillus thuringiensis known as phosphate-solubilizing bacteria were identified through 16S rRNA gene sequencing. After that, three sets of bio-fertilizers were randomly formulated. Each set consisted of nitrogen-fixing bacteria, potassium- and phosphate-solubilizing bacteria, as well as commercial compost as a carrier. These formulated bio-fertilizers were evaluated for plant growth promotion and protein production on duckweed plants under temperatures between 26 and 30°C. The results showed that each set of our formulated bio-fertilizer can increase the nitrogen (N), phosphorus (P), and potassium (K), duckweed growth, and protein content when compared to the control group. It indicates that bio-fertilizers formulated with mangrove-associated bacteria and high NPK contents could enhance the growth of duckweed as well as its protein content, which could supply our future plant-based protein sustainably.

Duckweed, a small flowering plant which comes from Lemnaceae family, has been rising in popularity to many researches and applications for its numerous valuable functional properties. One of its special features is fast growing plant and easily adapted to various types of regions which makes it attractive to be manipulated in many applications including in biofuel production, waste water treatment and also for pharmaceutical and medicinal purposes. Pectin is the common component found in plant cell wall and it has been used as food additive for its capability to gel, emulsify and stabilize food products. Several requirements for commercial pectin such as galacturonic acid content (GalA) and degree of amidation have been regulated. Studies have proven that duckweed have significant amount of pectin which make it as a potential source of gelling agent in food industry. However, the structural properties of pectin from duckweed need to be investigated, in order to ensure if they meet the regulated requirements for industrial production. It is also to determine the suitability of duckweed pectin utilization in different type of food products since pectin of different sources have different structural characteristics, thus exhibit different gelling capability. This present review discusses on the potential of pectin from duckweed species to be utilized as food additive with gelling function in food products. Several extraction methods also have been reviewed, which each of them showed different efficiency and affect the extracted pectin characteristics.

Plasenta haiwan merupakan salah satu bahan yang dimanfaatkan dalam produk kosmetik dan farmaseutikal, lantaran kandungan kimianya yang berkhasiat tinggi untuk tujuan tersebut. Pelanggan Muslim merupakan antara pengguna produk-produk yang diformulasi daripada plasenta tanpa mengambil peduli mengenai hukum penggunaan sama ada halal atau haram. Isunya ialah adakah plasenta termasuk dalam kategori organ haiwan yang halal digunakan ataupun tidak dalam Islam. Justeru, objektif artikel ini adalah bagi mengenalpasti status plasenta menurut hukum Islam dan menganalisis fatwa mengenainya dalam aspek halal dan haram. Metodologi kajian ini menggunakan pendekatan kualitatif, menerusi kaedah analisis dokumen. Analisis hukum plasenta diklasifikasikan mengikut sumber dari mana sesuatu plasenta diperoleh iaitu samada haiwan halal yang telah disembelih mengikut kaedah syarak, haiwan yang masih hidup, dan haiwan telah mati. Hasil kajian menunjukkan hanya plasenta yang berasal daripada haiwan halal dan telah disembelih secara syarak diiktiraf sebagai halal dan suci. Justeru, tiada halangan untuk dimanfaatkan untuk tujuan perubatan mahupun kosmetik. Manakala plasenta diambil daripada haiwan hidup atau telah mati tanpa disembelih adalah haram

DNA identification method is indispensable for the detection of a plant pathogen. However, established techniques, though reliable, requires advanced equipment, and their application outside specialized laboratories is limited. Along with the advancement of molecular techniques, several isothermal amplification methods, including Recombinase Polymerase Amplification (RPA), has been developed in this study. In fact, RPA is a rapid and sensitive amplification method, operating optimally at 37-42 degree celcius for 15 to 30 minutes with minimal sample preparation, and can amplify as low as 1-10 target copies. Furthermore, RPA has been a favourable method for the detection of plant pathogens due to its advantageous parameters. This review presents the current knowledge of RPA and its application in plant pathogen detection.

In recent years there has been a rapid growth of the halal pharmaceutical industry, especially in the supply chain of solid oral dosage forms of medication. This article outlines aspects of the Halal Management System (HMS) in the development and production of halal pharmaceuticals. It explains the needs and requirements of HMS and identifies the challenges faced in implementation. The article outlines aspects of executions and hurdles encountered when standardizing halal certifi cation. The article also highlights the need for systematic traceability systems and effective product recall mechanisms to ensure adherence to halal requirements. It also highlights the grey areas for halal in terms of pharmaceutical manufacture that are brought about by use of non-halal raw materials, e.g. alcohol, gelatine, glycerin, lecithin, glutamic acid and stearates.