Browsing by Author "Nur Amalina Mohd Amin"
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Publication Enhancement of Candida Rugosa Lipase Catalytic efficacy via immobilization onto selected organic and inorganic supports(Universiti Sains Islam Malaysia, 2013-09)Nur Amalina Mohd AminIn this study, Na-feldspar, nanoclay (Cloisite®30B) and kenaf core fibre have been used as enzyme immobilization supports. Through the enzyme screening procedure in the synthesis of butyl oleate, highest specific activity was acquired from Candida rugosa lipase (CRL) with 1.948 x 10-4 mmol ester/min/mg of protein compared to Candida antarctica, Candida cylindracea, Mucor javanicus, Mucor miehei and Aspergillus niger. Similarly, CRL exhibited the highest conversion of butyl oleate which was about 86.80%, thus it was selected as biocatalyst for further characterizations to suite industrial applications. Prior to immobilization, the physicochemical properties of selected support materials for lipase immobilization (Na-feldspar, nanoclay Cloisite®30B, kenaf core fibre) were characterized by using Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray (EDX), X-Ray Diffraction (XRD) and Physisorption of Nitrogen. Immobilization of CRL onto the supports via physical adsorption method showed that the highest percentage of immobilization was achieved in nanoclay (Cloisite®30B) (58.47%), followed by kenaf core fibre (53.39%) and Na-feldspar (12.07%). In the same way, nanoclay (Cloisite®30B) contained the highest protein loaded of 45.30 mg protein/g support, followed by kenaf core fibre and Na-feldspar, 36.71 mg protein/g support and 7.55 mg protein/g support, respectively. In adsorption isotherms analyses, nanoclay (Cloisite®30B) showed a sharp and steep slope at the lower concentration range, and a flat ‘plateau’ at the high range which was best described by the Langmuir isotherm. Kenaf core fibre and Na-feldspar systems however, showed typical characteristics of Freundlich isotherm with a ‘step’ or two plateaux over the Ce range of 60 protein mg/ml solution. Characterization of the immobilized lipases using SEM showed substantial, thick and rough external surface due to existence of lipase. Meanwhile, black spots of the lipases confined on the surface of supports were observed using TEM. The fingerprint elements of CRL and the supports were identified in EDX analysis. Successful immobilization of CRL onto supports was observed through dissimilarities in basal spacings shown by the XRD patterns and the significant differences in the values of surface area, pore diameter and pore volume between the supports and immobilized lipases obtained through the physisorption of nitrogen analysis. In screening of immobilized enzymes with high activities in the synthesis of butyl oleate, Nanoclay-CRL and Kenaf-CRL showed higher catalytic activity with 6.32 x 10-4 and 4.99 x 10-4 mmol/min/mg of protein, respectively. Optimal butyl oleate production (>80%) was achieved using Nanoclay-CRL and Kenaf-CRL after 12 hours of reactions. Nanoclay-CRL and Kenaf-CRL also showed high thermal stabilities with more than 85% retained relative activities after incubation at 70 oC. Nanoclay-CRL and Kenaf-CRL were also able to retain high activities and took over 16 to 19 cycles, to reach 50% of ester conversion. In the kinetic study of native and immobilized CRL, the lower Km(But) value suggested that the Nanoclay-CRL displayed higher affinity towards butanol (But) than towards oleic acid (Ol), Km(Ol) > Km(But). However, Native-CRL and Kenaf-CRL exhibited higher affinity towards oleic acid than towards butanol, Km(Ol) < Km(But). Despite of that, the Vmax(But) value showed that the initial rate catalyzed by Native-CRL in reactions containing varying butanol concentrations was higher than those catalyzed by Nanoclay-CRL and Kenaf-CRL. The immobilized lipases also demonstrated considerably higher Vmax(Ol) compared to Native-CRL in reactions containing varying oleic acid concentrations. The experimental findings of one substrate (Nanoclay-CRL and Kenaf-CRL) and two substrates (Native-CRL) competitive inhibition were best explained by the Ping-Pong Bi-Bi mechanism. - Some of the metrics are blocked by yourconsent settings
Publication Optimization of the Biodiesel Production via Transesterification Reaction of Palm Oil using Response Surface Methodology (RSM) A Review(USIM Press, 2022) ;Nur Aishah Rajali ;Salina Mat Radzi ;Maryam Mohamed RehanNur Amalina Mohd AminThe optimization method is vital in chemical synthesis and has been applied in many fields nowadays. Response surface methodology (RSM) is an example of an optimization method that is useful in examining the effects of multiple independent variables. RSM was applied in many studies to optimize the transesterification of biodiesel production from palm oil in the presence of a catalyst. This paper aims to provide an overview of recent catalyzed transesterification trends, as well as the benefits and drawbacks of heterogeneous, homogeneous, and enzyme catalysts in biodiesel production. RSM was used to design the process and statistically analyze the interaction effects of the independent reaction variables. The reaction variables, such as reaction time, reaction temperature, catalyst amount, and the molar ratio of the substrate, were optimized during the process. A statistical model and response surface plots were visualized graphically in the contour plots and three-dimensional figures to explain the interactive effects of variables on a response. In sum, this paper discussed the relationships between the reaction parameters and the production of biodiesel and the optimum conditions for biodiesel production using RSM. - Some of the metrics are blocked by yourconsent settings
Publication Optimization of the Biodiesel Production via Transesterification Reaction of Palm Oil using Response Surface Methodology (RSM): A Review(Institut Kimia Malaysia, 2023) ;Nur Aishah Rajali ;Salina Mat Radzi ;Nur Amalina Mohd AminMaryam Mohd RehanThe optimization method is vital in chemical synthesis and has been applied in many fields nowadays. Response surface methodology (RSM) is an example of an optimization method that is useful in examining the effects of multiple independent variables. RSM was applied in many studies to optimize the transesterification of biodiesel production from palm oil in the presence of a catalyst. This paper aims to provide an overview of recent catalyzed transesterification trends, as well as the benefits and drawbacks of heterogeneous, homogeneous, and enzyme catalysts in biodiesel production. RSM was used to design the process and statistically analyze the interaction effects of the independent reaction variables. The reaction variables such as reaction time, reaction temperature, catalyst amount, and the molar ratio of the substrate, were optimized during the process. A statistical model and response surface plots were visualized graphically in the contour plots and threed imensional figures to explain the interactive effects of variables on a response. In sum, this paper discussed the relationships between the reaction variables and the biodiesel production and the optimum conditions for biodiesel production using RSM. - Some of the metrics are blocked by yourconsent settings
Publication A Review on Cosmetic Formulations and Physicochemical Characteristics of Emollient and Day Cream Using Vegetable Based-Wax Ester(USIM Press, 2022) ;Nooratiqah Azmi ;Salina Mat Radzi ;Maryam Mohamed RehanNur Amalina Mohd AminThe cosmetic sector is one of the fastest-growing industries and continuously evolving as new technology is established. Skincare, haircare, make-up, and body care items were among the products available. Cosmetic formulas have progressed in recent years, allowing for the creation of more beneficial cosmetics. In chemistry, solids, liquids, and gases are primarily physicochemical characteristics. These are typically determined by a density, refractive index (RI), oxidation state, and vibrational frequency measurement. Physical and chemical qualities show a distinct feature that aids in the formulation of an enhanced emollient and day cream. This paper focuses on physicochemical characteristics such as RI, saponification value (SV), iodine value (IV), and acid value (AV). As for this review, the use of synthesised vegetable-based wax ester was utilised to formulate emollient and day cream. The esterification of vegetable-based waxes was performed by synthesising oleic acid from vegetable-based products and oleyl alcohol in the solvent.