The use of information and communication technology in the world of Education continues to develop various strategies and patterns. In higher education, e-learning is gaining more impact in teaching and learning that can be practiced in many ways. The advantages of developing and implementing e-learning in education increased learning effectiveness and flexibility, and much easier for teachers and students. Biodiversity is one of the Biology’s topic that need a lot of reading to be memorize by students. This innovation aims to develop a web-based system by using wix.com known as Interactive Biodiversity with INAQ (i- BioDi). INAQ is the Integration of Naqli and Aqli, also included to the related contents with the interactive learning memorizing tools such as mind maps, videos, and games. This website will be used as a hub for the biology subject for foundation students in Universiti Sains Islam Malaysia (USIM). While we acknowledge the importance of memorizing at this level of study, we have tried to focus our approach more toward understanding and explaining the rationale underlying many concepts of Biology. This website can also become a suitable reference for Malaysian Matriculation Programme, Sijil Tinggi Persekolahan Malaysia (STPM), and A-Level as well as any other pre-university Biology course. In this innovation, the Rapid Application Development was adapted as the development methodology. By using this interactive learning website, it will be more enjoyment and can helps the students to memorize using the interactive learning tools, learn and study the contents of the topic with element of INAQ.