This article describes a sensitive impedimetric method for the determination of human blood coagulation factor IX protein (FIX) which is present in extremely low concentration in serum. An interdigitated electrode (IDE) whose surface was layered with zinc oxide was modified with two kinds of probes. One is an antibody, the other an aptamer against FIX. A comparative study between anti-FIX aptamer and anti-FIX antibody showed the aptamer to possess higher affinity for FIX. A sandwich aptamer assay was worked out by using the FIX-binding aptamer on the surface of the IDE. It has a detection limit as low as 10 pM which makes it 4 to 30-fold more sensitive than any other method reported for FIX. Moreover, to practice detection in clinical samples, FIX was detected from the human blood serum by spiking. In our perception, the sensitivity of the ZnO-modified IDE presented here makes it a promising tool for sensing clinically relevant analytes that are present in very low (sub-pM) concentrations.

Diagnostic tools such as Point-of-Care (POC) analysis usually requires an on-site testing and measurements. Hence, to ease the fluid sample handling, microfluidics technology has been opted frequently to be incorporated with lab-on-chip (LOC). Therefore, an easy and conventional method of design and fabrication of the microfluidic device implemented. In this study, the device is fabricated with Polydimethylsiloxane (PDMS) and SU-8 using soft lithography technique. Herein, we focus on fluid transportation which is applied in clinical diagnostics and the transfer rate to the desired position as well as the fluid leakage during the transportation. Hence, the microfluidic sample transport channeling provides a practical solution for POC technological advances to study the biological systems that yield a better result in quantitative measurements and providing researchers new tools for life science research.

‘Janus particle’ refers the generation of two particles in single system having different physical properties and two surfaces involved in the formation of Janus are having different chemistries. In this study, we created the Janus using a hybrid of Zinc Oxide (ZnO) and gold (Au) on the sensor surface towards making a high-performance DNA sensor. In the current work, we present a novel solution process which involve single-step process to synthesize Janus ZnO/Au textured film using sol-gel method.

The authors describe an electrochemical method for the determination of the blood clotting factor IX (FIX). A nanogapped dielectrode (with a < 100 nm junction) was modified with antibody against FIX, and the resulting system was characterized by both impedance spectroscopy and voltammetry. In order to attain the improved sensitivity, gold nanoparticles were electrostatically attached to FIX. The current to voltage (I-V) measurement was carried out from 1 V to 5 V, where the entire calibration plot with 5 V was taken. This results in a limit of detection as low of 1 pM, which is much lower compared to the real concentration of FIX(87 nM) in human blood serum. The analytical range extends from 1 pM to 0.1 mu M. The electrode is highly specific over other serum proteins.