Browsing by Author "Rahman M.F."
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Publication Assessing resistance and bioremediation ability of enterobacter sp. Strain saw-1 on molybdenum in various heavy metals and pesticides(Institute for Research and Community Services, Institut Teknologi Bandung, 2017) ;Sabullah M.K. ;Rahman M.F. ;Ahmad S.A. ;Sulaiman M.R. ;Shukor M.S. ;Shamaan N.A. ;Shukor M.Y. ;Faculty of Medicine and Health Sciences ;Universiti Malaysia Sabah (UMS) ;Universiti Putra Malaysia (UPM) ;Snoc International Sdn BhdUniversiti Sains Islam Malaysia (USIM)One of the most economical approaches for removal of toxic compounds is bioremediation. In the long term, bioremediation is economic and feasible compared to other methods, such as physical or chemical methods. A bacterium that can efficiently reduce molybdenum blue was isolated from polluted soil. Biochemical analysis revealed the identity of the bacterium as Enterobacter sp. strain Saw-1. The growth parameters for optimal reduction of molybdenum to Mo-blue or molybdenum blue, a less toxic product, were determined around pH 6.0 to 6.5 and in the range of 30 to 37 �C, respectively. Glucose was selected as preferred carbon source, followed by sucrose, maltose, l-rhamnose, cellobiose, melibiose, raffinose, d-mannose, lactose, glycerol, d-adonitol, d-mannitol, l-arabinose and mucate. Phosphate and molybdate were critically required at 5.0 mM and 10 mM, respectively. The scanning absorption spectrum acquired to detect the development of complex Mo-blue showed similarity to previously isolated Mo-reducing bacteria. In addition, the spectrum closely resembled the molybdenum blue from the phosphate determination method. Heavy metals, including mercury, copper (II) and silver (I), inhibited reduction. Moreover, the bacterium also showed capability of exploiting the pesticide coumaphos as an alternative carbon source for growth. As the bacterium proved its ability to detoxify organic and inorganic xenobiotics, the usefulness of this microorganism for bioremediation is highlighted. � 2017 Published by ITB Journal Publisher. - Some of the metrics are blocked by yourconsent settings
Publication Isolation and characterization of a molybdenum-reducing and glyphosate-degrading klebsiella oxytoca strain saw-5 in soils from sarawak(Agriculture Faculty Brawijaya University, 2016) ;Sabullah M.K. ;Rahman M.F. ;Ahmad S.A. ;Sulaiman M.R. ;Shukor M.S. ;Shamaan N.A. ;Shukor M.Y. ;Universiti Malaysia Sabah (UMS) ;Universiti Putra Malaysia (UPM) ;Snoc International Sdn BhdUniversiti Sains Islam Malaysia (USIM)Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molyb-denum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best elec-tron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spec-trum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants. � 2016, Agriculture Faculty Brawijaya University. All rights reserved. - Some of the metrics are blocked by yourconsent settings
Publication Isolation and characterization of a molybdenum-reducing and phenolic- and catechol-degrading enterobacter sp. Strain saw-2(Seameo Biotrop, 2017) ;Sabullah M.K. ;Rahman M.F. ;Ahmad S.A. ;Sulaiman M.R. ;Shukor M.S. ;Gansau A.J. ;Shamaan N.A. ;Shukor M.Y. ;Faculty of Medicine and Health Sciences ;Universiti Malaysia Sabah (UMS) ;Universiti Putra Malaysia (UPM)Universiti Sains Islam Malaysia (USIM)Molybdenum is an emerging pollutant worldwide. The objective of this study is to isolate molybdenum-reducing bacterium with the ability to grow on phenolic compounds (phenol and catechol). The screening process was carried out on a microplate. The bacterium reduced molybdenum in the form of sodium molybdate to molybdenum blue (Mo-blue). The bacterium required a narrow pH range for optimal reduction of molybdenum, i.e. Between pH 6.3 and 6.8, with temperature between 34 and 37 o C. Molybdate reduction to Mo-blue was best supported by glucose as the carbon source. However, both phenol and catechol could not support molybdate reduction. Other requirements for molybdate reduction included sodium molybdate concentrations between 15 and 30 mM, and phosphate concentration of 5.0 mM. The bacterium exhibited a Mo-blue absorption spectrum with a shoulder at 700 nm and a maximum peak near the infrared region at 865 nm. The Mo-reducing bacterium was partially identified as Enterobacter sp. Strain Saw-2. The capability of this bacterium to grow on toxic phenolic compounds and to detoxify molybdenum made it a significant agent for bioremediation.