Browsing by Author "Rouzheinikov S.N."

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    Publication
    Crystallization and preliminary crystallographic analysis of a surface antigen glycoprotein, SAG19, from Eimeria tenella
    (2013)
    Ramly N.Z.
    ;
    Rouzheinikov S.N.
    ;
    Sedelnikova S.E.
    ;
    Baker P.J.
    ;
    Chow Y.-P.
    ;
    Wan K.-L.
    ;
    Nathan S.
    ;
    Rice D.W.
    ;
    Faculty of Science and Technology
    ;
    University of Sheffield
    ;
    Universiti Sains Islam Malaysia (USIM)
    ;
    Universiti Kebangsaan Malaysia (UKM)
    ;
    Malaysia Genome Institute
    Coccidiosis in chickens is caused by the apicomplexan parasite Eimeria tenella and is thought to involve a role for a superfamily of more than 20 cysteine-rich surface antigen glycoproteins (SAGs) in host-parasite interactions. A representative member of the family, SAG19, has been overexpressed in Escherichia coli, purified and crystallized by the hanging-drop method of vapour diffusion using ammonium sulfate as the precipitant. Crystals of SAG19 diffracted to beyond 1.50� resolution and belonged to space group I4, with unit-cell parameters a = b = 108.2, c = 37.5�. Calculation of possible values of V M suggests that there is a single molecule in the asymmetric unit. � 2013 International Union of Crystallography.