Browsing by Author "Shukor M.S."
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Publication AIsolation and Characterization of a Heavy Metalreducing Enterobacteriaceae Bacterium Strain DRY 7 with the Ability to Assimilate Phenol and Diesel(Indian Society for Education and Environment, 2016) ;Hamdan M.H. ;Othaman M.A. ;Suhaili Z. ;Shukor M.S. ;Halmi M.I.E. ;Abdullah S.R.S. ;Shamaan N.A. ;Shukor M.Y. ;Faculty of Medicine and Health Sciences ;Universiti Putra Malaysia (UPM) ;Universiti Sultan Zainal Abidin (UniSZA) ;Snoc International SdnBhd ;Universiti Kebangsaan Malaysia (UKM)Universiti Sains Islam Malaysia (USIM)Background/Objectives: Molybdenum, phenol and diesel are toxic to organism, and are part of global pollution. Their removal using microorganisms with multiple detoxification ability is being intensely sought as a cleaner and economic approach. Methods/Statistical analysis: A soil suspension was spread plated on a minimal salts media supplemented with molybdenum. Blue colonies, indicating molybdenum reduction was then screened for phenol and diesel degradation capabilities. Findings: A molybdenum-reducing bacterium locally isolated showed the ability to grow on phenol and diesel. The bacterium required pHs of between 5.8 and 6.3 and temperatures of between 30 and 40oC for optimal reduction. Among the carbon sources tested for supporting reduction, glucose was the best. A critical concentration of phosphate at just 5 mM was required, while molybdenum (sodium molybdate) was required between 15 and 25 mM. The absorption spectrum of the Mo-blue produced showed a characteristic maximum peak at 865 nm. The reduction of molybdenum was inhibited by the ions mercury, copper, chromium, lead and silver by 78.9, 78.4, 77.4, 53.5 and 36.8%, respectively. Analysis using phylogenetic analysis identifies the bacterium as Enterobacteriaceae bacterium strain DRY7. Growth on phenol and diesel as carbon sources showed that the optimal concentrations supporting growth was between 300 and 400 mg/L and between 300 and 500 mg/L, respectively. Application/Improvements: The capacity of this bacterium to detoxify a number of toxicants is an important property or bioremediation of soils contaminated with multiple toxicants. - Some of the metrics are blocked by yourconsent settings
Publication Assessing resistance and bioremediation ability of enterobacter sp. Strain saw-1 on molybdenum in various heavy metals and pesticides(Institute for Research and Community Services, Institut Teknologi Bandung, 2017) ;Sabullah M.K. ;Rahman M.F. ;Ahmad S.A. ;Sulaiman M.R. ;Shukor M.S. ;Shamaan N.A. ;Shukor M.Y. ;Faculty of Medicine and Health Sciences ;Universiti Malaysia Sabah (UMS) ;Universiti Putra Malaysia (UPM) ;Snoc International Sdn BhdUniversiti Sains Islam Malaysia (USIM)One of the most economical approaches for removal of toxic compounds is bioremediation. In the long term, bioremediation is economic and feasible compared to other methods, such as physical or chemical methods. A bacterium that can efficiently reduce molybdenum blue was isolated from polluted soil. Biochemical analysis revealed the identity of the bacterium as Enterobacter sp. strain Saw-1. The growth parameters for optimal reduction of molybdenum to Mo-blue or molybdenum blue, a less toxic product, were determined around pH 6.0 to 6.5 and in the range of 30 to 37 �C, respectively. Glucose was selected as preferred carbon source, followed by sucrose, maltose, l-rhamnose, cellobiose, melibiose, raffinose, d-mannose, lactose, glycerol, d-adonitol, d-mannitol, l-arabinose and mucate. Phosphate and molybdate were critically required at 5.0 mM and 10 mM, respectively. The scanning absorption spectrum acquired to detect the development of complex Mo-blue showed similarity to previously isolated Mo-reducing bacteria. In addition, the spectrum closely resembled the molybdenum blue from the phosphate determination method. Heavy metals, including mercury, copper (II) and silver (I), inhibited reduction. Moreover, the bacterium also showed capability of exploiting the pesticide coumaphos as an alternative carbon source for growth. As the bacterium proved its ability to detoxify organic and inorganic xenobiotics, the usefulness of this microorganism for bioremediation is highlighted. � 2017 Published by ITB Journal Publisher. - Some of the metrics are blocked by yourconsent settings
Publication Characterization of a molybdenum-reducing Bacillus sp. strain khayat with the ability to grow on SDS and diesel(Springer-Verlag Italia s.r.l., 2016) ;Khayat M.E. ;Rahman M.F.A. ;Shukor M.S. ;Ahmad S.A. ;Shamaan N.A. ;Shukor M.Y. ;Faculty of Medicine and Health Sciences ;Universiti Putra Malaysia (UPM) ;Snoc International Sdn BhdUniversiti Sains Islam Malaysia (USIM)Molybdenum and heavy metals are toxicants that are needed to be removed from the environment as they are non-biodegradable and pose a serious threat to the ecology. A previously isolated keratin-degrading Bacillus sp. strain khayat was shown to be able to reduce molybdenum (sodium molybdate) to molybdenum blue (Mo-blue). Reduction occurred optimally at the pHs of between 5.8 and 6.8 and temperatures of between 25 and 34��C. Glucose was the best electron donor for supporting molybdate reduction followed by sucrose, fructose, glycogen, lactose, meso-inositol and glycerol in descending order. Other requirements include a phosphate concentration between 5.0 and 7.5�mM and a molybdate concentration of between 10 and 20�mM. The absorption spectrum of the Mo-blue produced was similar to previous Mo-reducing bacterium, and closely resembles a reduced phosphomolybdate. Molybdenum reduction was inhibited by Hg (ii), Ag (i), Cu (ii), As (v) and Pb (ii) at 84.7, 78.9, 53.5, 36.8 and 27.7�%, respectively. Analysis using phylogenetic analysis resulted in a tentative identification of the bacterium as Bacillus sp. strain khayat. The bacterium was unable utilize any of the xenobiotics as sources of electron donor for reduction but the bacterium was able to grow on diesel and SDS. The ability of this bacterium to detoxify several toxicants makes this bacterium an important tool for bioremediation of multiple toxicants. � 2016, Accademia Nazionale dei Lincei. - Some of the metrics are blocked by yourconsent settings
Publication Isolation and characterization of a molybdenum-reducing and glyphosate-degrading klebsiella oxytoca strain saw-5 in soils from sarawak(Agriculture Faculty Brawijaya University, 2016) ;Sabullah M.K. ;Rahman M.F. ;Ahmad S.A. ;Sulaiman M.R. ;Shukor M.S. ;Shamaan N.A. ;Shukor M.Y. ;Universiti Malaysia Sabah (UMS) ;Universiti Putra Malaysia (UPM) ;Snoc International Sdn BhdUniversiti Sains Islam Malaysia (USIM)Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molyb-denum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best elec-tron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spec-trum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants. � 2016, Agriculture Faculty Brawijaya University. All rights reserved. - Some of the metrics are blocked by yourconsent settings
Publication Isolation and characterization of a molybdenum-reducing and phenolic- and catechol-degrading enterobacter sp. Strain saw-2(Seameo Biotrop, 2017) ;Sabullah M.K. ;Rahman M.F. ;Ahmad S.A. ;Sulaiman M.R. ;Shukor M.S. ;Gansau A.J. ;Shamaan N.A. ;Shukor M.Y. ;Faculty of Medicine and Health Sciences ;Universiti Malaysia Sabah (UMS) ;Universiti Putra Malaysia (UPM)Universiti Sains Islam Malaysia (USIM)Molybdenum is an emerging pollutant worldwide. The objective of this study is to isolate molybdenum-reducing bacterium with the ability to grow on phenolic compounds (phenol and catechol). The screening process was carried out on a microplate. The bacterium reduced molybdenum in the form of sodium molybdate to molybdenum blue (Mo-blue). The bacterium required a narrow pH range for optimal reduction of molybdenum, i.e. Between pH 6.3 and 6.8, with temperature between 34 and 37 o C. Molybdate reduction to Mo-blue was best supported by glucose as the carbon source. However, both phenol and catechol could not support molybdate reduction. Other requirements for molybdate reduction included sodium molybdate concentrations between 15 and 30 mM, and phosphate concentration of 5.0 mM. The bacterium exhibited a Mo-blue absorption spectrum with a shoulder at 700 nm and a maximum peak near the infrared region at 865 nm. The Mo-reducing bacterium was partially identified as Enterobacter sp. Strain Saw-2. The capability of this bacterium to grow on toxic phenolic compounds and to detoxify molybdenum made it a significant agent for bioremediation.