Browsing by Author "Suhaili Z."
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Publication AIsolation and Characterization of a Heavy Metalreducing Enterobacteriaceae Bacterium Strain DRY 7 with the Ability to Assimilate Phenol and Diesel(Indian Society for Education and Environment, 2016) ;Hamdan M.H. ;Othaman M.A. ;Suhaili Z. ;Shukor M.S. ;Halmi M.I.E. ;Abdullah S.R.S. ;Shamaan N.A. ;Shukor M.Y. ;Faculty of Medicine and Health Sciences ;Universiti Putra Malaysia (UPM) ;Universiti Sultan Zainal Abidin (UniSZA) ;Snoc International SdnBhd ;Universiti Kebangsaan Malaysia (UKM)Universiti Sains Islam Malaysia (USIM)Background/Objectives: Molybdenum, phenol and diesel are toxic to organism, and are part of global pollution. Their removal using microorganisms with multiple detoxification ability is being intensely sought as a cleaner and economic approach. Methods/Statistical analysis: A soil suspension was spread plated on a minimal salts media supplemented with molybdenum. Blue colonies, indicating molybdenum reduction was then screened for phenol and diesel degradation capabilities. Findings: A molybdenum-reducing bacterium locally isolated showed the ability to grow on phenol and diesel. The bacterium required pHs of between 5.8 and 6.3 and temperatures of between 30 and 40oC for optimal reduction. Among the carbon sources tested for supporting reduction, glucose was the best. A critical concentration of phosphate at just 5 mM was required, while molybdenum (sodium molybdate) was required between 15 and 25 mM. The absorption spectrum of the Mo-blue produced showed a characteristic maximum peak at 865 nm. The reduction of molybdenum was inhibited by the ions mercury, copper, chromium, lead and silver by 78.9, 78.4, 77.4, 53.5 and 36.8%, respectively. Analysis using phylogenetic analysis identifies the bacterium as Enterobacteriaceae bacterium strain DRY7. Growth on phenol and diesel as carbon sources showed that the optimal concentrations supporting growth was between 300 and 400 mg/L and between 300 and 500 mg/L, respectively. Application/Improvements: The capacity of this bacterium to detoxify a number of toxicants is an important property or bioremediation of soils contaminated with multiple toxicants. - Some of the metrics are blocked by yourconsent settings
Publication Comparative study of physico-chemical analyses of different water resources in Setiu Wetland, Terengganu(Springer International Publishing, 2018) ;Lani M.N. ;Malek Rivan N.F. ;Ismail A. ;Wan Omar W.B. ;Hassan Z. ;Suhaili Z. ;Faculty of Science and Technology ;Universiti Malaysia Terengganu (UMT) ;Universiti Sains Islam Malaysia (USIM)Universiti Sultan Zainal Abidin (UniSZA)Physico-chemical properties of water are usually reflecting the functioning of an aquatic ecosystem like Setiu Wetland, Terengganu. Therefore, this study was conducted to determine the physico-chemical properties and chemical nature of their water resources (trench, rivers, drainage and tap water) and their impact on sustainable water resource in Setiu Wetland, Terengganu. A total of 12 water samples were collected aseptically from in this area and the field work was conducted from August to September 2015. In-situ measurements of physico-chemical properties of the water samples were recorded using YSI Professional Plus Parameter (XYLEM, USA) with multi-sensor probe. The analyses of physico-chemical of water include of water temperature, barometric pressure, dissolved oxygen, specific conductance, conductance, redox potential, total suspended solids, salinity and pH. In comparison to previous study conducted in 2008, most of the parameters showed that water quality at Setiu Wetland, Terengganu remained Class 1 based on National Water Quality Standards for Malaysia. In conclusion, the various water resources in Setiu Wetland was in very good condition indicating that the habitats in Setiu Wetlands have conditions suitable to support a biotic community. Further study should be carried out in monitoring water qualities of the water resources at Malaysia to increase safety of water consumption. � Springer Nature Switzerland AG 2019. - Some of the metrics are blocked by yourconsent settings
Publication Microbiological evaluation on raw materials and food contact surfaces of ‘keropok lekor’ premises in Kuala Nerus, Terengganu and their prevalence of antibiotic resistant bacteria(Malaysian Society of Applied Biology, 2017) ;Lani M.N. ;Peng T.A. ;Suhaili Z. ;Hassan Z. ;Faculty of Science and Technology ;Universiti Malaysia Terengganu (UMT) ;Universiti Sultan Zainal Abidin (UniSZA)Universiti Sains Islam Malaysia (USIM)‘Keropok lekor’ is a popular street food and widely available in Terengganu. However, there is limited study on the microbiological status of sources of contamination from ‘keropok lekor’ premises in Terengganu. Microbiological quality of raw materials and food contact surfaces were determined by Total Plate Count, coliform count, Staphylococcus spp. and Vibrio spp. count. The presumptive of Escherichia coli, Staphylococcus aureus and Vibrio spp. were further identified by phenotypic identification such as IMViC tests and API20 NE identification system. The antibiotic resistance of the identified bacteria was determined using disc diffusion method. Escherichia coli and Vibrio were predominant microorganisms isolated from raw materials, whereas Staphylococcus was the predominant microorganism on food contact surfaces. The boiling process of ‘keropok lekor’ at 100°C was significantly (p < 0.05) reduced the Total Plate Counts, coliform, Staphylococcus spp. and Vibrio spp. count for safe consumption. The identified antibiotic resistance of E. coli, S. aureus and Vibrio spp. showed that the bacteria were within controllable emergence strains, whereby the present antibiotics used are able to suppress the growth. Regular monitoring programme is essential to further improve the microbiological quality of raw materials and food contact surfaces.