This brief study examines the science of Quranic script derived from the manuscripts of various regions, starting from the systematic work undertaken by the Rightly Guided Caliph Uthman ibn Affan (may Allah be pleased with him) until it matured into a well-established discipline. It explores how this science influenced ancient Quranic manuscripts and aims to elucidate the technical and scientific specifications of the Quranic scripts from different regions and their impact on ancient manuscripts. The study is organized into three sections, preceded by an introduction and followed by a conclusion and results. The analytical method was employed to present its issues. The study reached significant conclusions, including that the books on Quranic script are considered reliable references in describing the scriptural phenomena of regional Quranic manuscripts and are the primary sources for judging the characteristics of ancient Quranic manuscripts.