Publication:
Comparison of Real-Time PCR and FTIR with Chemometrics Technique in Analysing Halal Supplement Capsules

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dc.contributor.authorMohd Sukri Hassanen_US
dc.contributor.authorAhlam Inayatullah Badrul Muniren_US
dc.contributor.authorM. Husaini A. Rahmanen_US
dc.date.accessioned2024-05-28T04:28:00Z
dc.date.available2024-05-28T04:28:00Z
dc.date.issued2017
dc.descriptionVolume:12 No: 9en_US
dc.description.abstractHalal authentication and verification in supplement capsules are highly required as the gelatine available in the market can be from halal or non-halal sources. It is an obligation for Muslim to consume and use the halal consumer goods. At present, real-time polymerase chain reaction (RT-PCR) is the most common technique being used for the detection of porcine and bovine DNA in gelatine due to high sensitivity of the technique and higher stability of DNA compared to protein. In this study, twenty samples of supplements capsules from different products with different Halal logos were analyzed for porcine and bovine DNA using RT-PCR. Standard bovine and porcine gelatine from eurofins at a range of concentration from 10-1 to 10-5 ng/?l were used to determine the linearity range, limit of detection and specificity on RT-PCR (SYBR Green method). RT-PCR detected porcine (two samples), bovine (four samples) and mixture of porcine and bovine (six samples). The samples were also tested using FT-IR technique where normalized peak of IR spectra were pre-processed using Savitsky Golay method before Principal Components Analysis (PCA) was performed on the database. Scores plot of PCA shows three clusters of samples; bovine, porcine and mixture (bovine and porcine). The RT-PCR and FT-IR with chemometrics technique were found to give same results for porcine gelatine samples which can be used for Halal authentication.en_US
dc.identifier.doi10.5281/zenodo.1474717
dc.identifier.doihttps://zenodo.org/record/1474717#.YambYVVBzcs
dc.identifier.epage320
dc.identifier.issue9
dc.identifier.other1632-11
dc.identifier.spage316
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/5698
dc.identifier.volume12
dc.language.isoen_USen_US
dc.publisherWorld Academy of Science, Engineering and Technologyen_US
dc.relation.ispartofInternational Journal of Nutrition and Food Engineeringen_US
dc.subjectHalal, real-time PCR, gelatin, FTIR, chemometricsen_US
dc.titleComparison of Real-Time PCR and FTIR with Chemometrics Technique in Analysing Halal Supplement Capsulesen_US
dc.typeArticleen_US
dspace.entity.typePublication

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