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Effects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cells

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dc.Chemicals/CASinvolucrin, 60108-77-2; protein p21, 85306-28-1; recombinant epidermal growth factor, 62253-63-8; Epidermal Growth Factor, 62229-50-9; Mitogens; Transcription Factors
dc.FundingDetailsUniversiti Kebangsaan Malaysia Ministry of Science, Technology and Space: 02-01-02-SF0289
dc.FundingDetailsThis study was funded by the Ministry of Science, Technology and Innovations , Grant number: 02-01-02-SF0289 . Simat Siti Fatimah is our PhD student in Universiti Kebangsaan Malaysia and is a National Science Fellowship scholar of Ministry of Science, Technology and Innovations.
dc.ManufacturersPeproTech, United States
dc.MolecularSequenceNumbersGENBANK: NM_000321, NM_000526, NM_000546, NM_001975, NM_002046, NM_002374, NM_002417, NM_002577, NM_002701, NM_003106, NM_003722, NM_005382, NM_005547, NM_006121, NM_024865, NM_052850, NM_053056
dc.citedby17
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorFatimah S.S.en_US
dc.contributor.authorTan G.C.en_US
dc.contributor.authorChua K.H.en_US
dc.contributor.authorTan A.E.en_US
dc.contributor.authorHayati A.R.en_US
dc.date.accessioned2024-05-29T01:58:34Z
dc.date.available2024-05-29T01:58:34Z
dc.date.issued2012
dc.description.abstractHuman amnion epithelial cells (HAECs) hold great promise in tissue engineering for regenerative medicine. Large numbers of HAECs are required for this purpose. Hence, exogenous growth factor is added to the culture medium to improve epithelial cells proliferation. The aim of the present study was to determine the effects of epidermal growth factor (EGF) on the proliferation and cell cycle regulation of cultured HAECs. HAECs at P1 were cultured for 7 days in medium containing an equal volume mix of HAM's F12: Dulbecco's Modified Eagles Medium (1:1) supplemented with different concentrations of EGF (0, 5, 10, 20, 30 and 50. ng/ml EGF) in reduced serum. Morphology, growth kinetics and cell cycle analysis using flow cytometry were assessed. Quantitative gene expression for cell cycle control genes, pluripotent transcription factors, epithelial genes and neuronal genes were also determined. EGF enhanced HAECs proliferation with optimal concentration at 10. ng/ml EGF. EGF significantly increased the proportion of HAECs at S- and G2/M-phase of the cell cycle compared to the control. At the end of culture, HAECs remained as diploid cells under cell cycle analysis. EGF significantly decreased the mRNA expression of p21, pRb, p53 and GADD45 in cultured HAECs. EGF also significantly decreased the pluripotent genes expression: Oct-3/4, Sox2 and Nanog; epithelial genes expression: CK14, p63, CK1 and Involucrin; and neuronal gene expression: NSE, NF-M and MAP 2. The results suggested that EGF is a strong mitogen that promotes the proliferation of HAECs through cell cycle regulation. EGF did not promote HAECs differentiation or pluripotent genes expression. � 2012 The Society for Biotechnology, Japan.en_US
dc.description.natureFinalen_US
dc.identifier.CODENJBBIF
dc.identifier.doi10.1016/j.jbiosc.2012.03.021
dc.identifier.epage227
dc.identifier.issn13891723
dc.identifier.issue2
dc.identifier.pmid22578596
dc.identifier.scopus2-s2.0-84863208349
dc.identifier.spage220
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84863208349&doi=10.1016%2fj.jbiosc.2012.03.021&partnerID=40&md5=3c7ca4c45a3e0c5460cd919d5ef4d511
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10008
dc.identifier.volume114
dc.languageEnglish
dc.language.isoen_USen_US
dc.relation.ispartofJournal of Bioscience and Bioengineering
dc.sourceScopus
dc.subjectCell cycleen_US
dc.subjectEpidermal growth factoren_US
dc.subjectEpithelialen_US
dc.subjectHuman amnionen_US
dc.subjectPluripotenten_US
dc.subjectTissue engineeringen_US
dc.titleEffects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cellsen_US
dc.title.alternativeJ. Biosci. Bioeng.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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