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Characterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides

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dc.contributor.authorAzman, Aida Rasyidahen_US
dc.contributor.authorMahat, Naji Arafaten_US
dc.contributor.authorWahab, Roswanira Abdulen_US
dc.contributor.authorAhmad, Wan Azlinaen_US
dc.contributor.authorHuri, Mohamad Afiq Mohameden_US
dc.contributor.authorHamid, Azzmer Azzar Abdulen_US
dc.contributor.authorAdamu, Aliyuen_US
dc.contributor.authorSaat, Geshina Ayu Maten_US
dc.date.accessioned2024-05-29T03:00:26Z
dc.date.available2024-05-29T03:00:26Z
dc.date.issued2020
dc.description.abstractConsidering the significant evidential values of fingerprints in underwater criminal investigations and the need to visualise them using a user- and environmentally-friendly reagent, development of a novel, rapid and relatively greener nanobio-based reagent (NBR) is deemed beneficial. Lipase from the commercial Candida rugosa immobilised onto acid-functionalised multi-walled carbon nanotubes (NBR) was used as the safer and cheap lipid-sensing reagent to visualise groomed whole/split fingerprints on non-porous objects immersed in stagnant tap water for up to 30 days under a laboratory-controlled setting. Attenuated Total Reflectance - Fourier Transform Spectrometry, Field Emission Scanning Electron Microscopy and bioinformatics (molecular docking and molecular dynamics simulations) were employed to characterise and confirm the attachment of NBR onto the lipid constituents of wet fingerprints. Chromatographic results further confirmed the presence of n-hex-adecanoic and octadecanoic acids on fingerprints up to 30 days of immersion. Thus, NBR may potentially be useful as the future state-of-the-art fingerprint visualisation technology.
dc.identifier.doi10.1016/j.procbio.2020.05.033
dc.identifier.epage112
dc.identifier.issn1359-5113
dc.identifier.scopusWOS:000551342900011
dc.identifier.spage102
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11782
dc.identifier.volume96
dc.languageEnglish
dc.language.isoen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofProcess Biochemistry
dc.subjectLatent fingerprinten_US
dc.subjectNanobio-based reagenten_US
dc.subjectCandida rugosa lipaseen_US
dc.subjectBioinformaticsen_US
dc.subjectForensic scienceen_US
dc.titleCharacterisation and computational analysis of a novel lipase nanobio-based reagent for visualising latent fingerprints on water-immersed glass slides
dc.typeArticleen_US
dspace.entity.typePublication

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