Publication: In Vitro And In Vivo Effects Of Puntius Javanicus Cholinesterase By Copper
| dc.contributor.author | Sabullah, MK | en_US |
| dc.contributor.author | Sulaiman, MR | en_US |
| dc.contributor.author | Abd Shukor, MY | en_US |
| dc.contributor.author | Shamaan, NA | en_US |
| dc.contributor.author | Khalid, A | en_US |
| dc.contributor.author | Ahmad, SA | en_US |
| dc.date.accessioned | 2024-05-29T03:25:22Z | |
| dc.date.available | 2024-05-29T03:25:22Z | |
| dc.date.issued | 2015 | |
| dc.description.abstract | The aim of this study is to determine the inhibitory effect of copper towards butyrylcholinesterase (BChE) activity. Using the Lineweaver-Burk plot, Puntius javanicus BChE activity was found to be noncompetitively inhibited by copper. The maximal velocities of untreated (control) BChE, 0.5 and 10 mg/L copper-treated BChE are 53.70, 31.81 and 14.30 Umg(-1), respectively, while the biomolecular constant (K-m) values of both tests shows no significant difference (p>0.05). The in vitro IC50 of copper ion to the BChE was found to be 0.0948 (0.0658 to 0.1691) mg/L. In vivo tests showed that in the presence of 0.1 mg/L copper, the BChE activity was slightly higher compared to the untreated control. Copper sulfate at 0.3 mg/L concentration showed no significant inhibition compared to control. However, the activity decreased with increasing copper concentrations of 0.5, 1.0 and 5.0 mg/L, with the remaining activity at 87.60, 84.60 and 73.00 %, respectively. This study suggests that BChE isolated from P. javanicus liver tissue is a potentially new source of biomarker for copper contamination. | |
| dc.identifier.epage | 4621 | |
| dc.identifier.isbn | 1610-2304 | |
| dc.identifier.issn | 1018-4619 | |
| dc.identifier.issue | 12B | |
| dc.identifier.scopus | WOS:000368040900009 | |
| dc.identifier.spage | 4615 | |
| dc.identifier.uri | https://oarep.usim.edu.my/handle/123456789/11947 | |
| dc.identifier.volume | 24 | |
| dc.language | English | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Parlar Scientific Publications (P S P) | en_US |
| dc.relation.ispartof | Fresenius Environmental Bulletin | |
| dc.source | Web Of Science (ISI) | |
| dc.subject | Butyrylcholinesterase (BChE) | en_US |
| dc.subject | P. javanicus | en_US |
| dc.subject | Copper | en_US |
| dc.title | In Vitro And In Vivo Effects Of Puntius Javanicus Cholinesterase By Copper | en_US |
| dc.title.alternative | Fresenius Environ. Bull. | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication |