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Vibrational Spectroscopy-Based Chemometrics Analysis of Clinacanthus nutans Extracts after Postharvest Processing and Extract Effects on Cardiac C-Kit Cells

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dc.contributor.authorVuanghao Limen_US
dc.contributor.authorHui Wen Chongen_US
dc.contributor.authorNozlena Abdul Samaden_US
dc.contributor.authorSiti Aisyah Abd Ghafaren_US
dc.contributor.authorIda Shazrina Ismailen_US
dc.contributor.authorRafeezul Mohameden_US
dc.contributor.authorYoke Keong Yongen_US
dc.contributor.authorChee Yuen Ganen_US
dc.contributor.authorJun Jie Tanen_US
dc.date.accessioned2024-05-28T05:59:31Z
dc.date.available2024-05-28T05:59:31Z
dc.date.issued2022
dc.date.submitted2022-7-26
dc.descriptionHindawi Evidence-Based Complementary and Alternative Medicine Volume 2022, Article ID 1967593, 11 pagesen_US
dc.description.abstractChemical constituents in plants can be greatly affected by postharvest processing, and it is important to identify the factors that lead to significant changes in chemistry and bioactivity. In this study, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy was used to analyze extracts of Clinacanthus nutan (C. nutans) leaves generated using different parameters (solvent polarities, solid-liquid ratios, ultrasonic durations, and cycles of extraction). In addition, the effects of these extracts on the viability of cardiac c-kit cells (CCs) were tested. The IR spectra were processed using SIMCA-P software. PCA results of all tested parameter sets were within acceptable values. Solvent polarity was identified as the most influential factor to observe the differences in chemical profile and activities of C. nutans extracts. Ideal extraction conditions were identified, for two sample groups (G1 and G2), as they showed optimal total phenolic content (TPC) yield of 44.66 ± 0.83 mg GAE/g dw and 45.99 ± 0.29 mg GAE/g dw and CC viability of 171.81 ± 4.06% and 147.53 ± 6.80%, respectively. Validation tools such as CV-ANOVA () and permutation (R2 and Q2 plots were well intercepted to each other) have further affirmed the significance and reliability of the partial least square (PLS) model of solvent polarity employed in extraction. Hence, these approaches help optimize postharvest processes that encourage positive TPC and CCs results in C. nutans extracts.en_US
dc.identifier.doi10.1155/2022/1967593
dc.identifier.epage11
dc.identifier.issn1741-427X
dc.identifier.issueNA
dc.identifier.other1815-23
dc.identifier.spage1
dc.identifier.urihttps://www.hindawi.com/journals/ecam/2022/1967593/
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/6917
dc.identifier.volume2022
dc.language.isoenen_US
dc.publisherHindawien_US
dc.relation.ispartofEvidence-Based Complementary and Alternative Medicineen_US
dc.titleVibrational Spectroscopy-Based Chemometrics Analysis of Clinacanthus nutans Extracts after Postharvest Processing and Extract Effects on Cardiac C-Kit Cellsen_US
dc.typeArticleen_US
dspace.entity.typePublication

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