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Characterization And Potential Oral Probiotic Properties Of Lactobacillus Plantarum Ft 12 And Lactobacillus Brevis Ft 6 Isolated From Malaysian Fermented Food

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dc.contributor.authorNurul Szawani Mohd-Zubrien_US
dc.contributor.authorKalavathy Ramasamyen_US
dc.contributor.authorNor Zaihana Abdul Rahmanen_US
dc.date.accessioned2024-05-29T01:54:55Z
dc.date.available2024-05-29T01:54:55Z
dc.date.issued2022
dc.date.submitted2023-2-9
dc.descriptionArchives of Oral Biology Volume 143, 105515 Page (1-8)en_US
dc.description.abstractObjective: This study aims to characterise the lactic acid bacteria (LAB) isolated from local Malaysian fermented foods with oral probiotics properties. Design: The LAB strains isolated from Malaysian fermented foods, Lactobacillus brevis FT 6 and Lactobacillus plantarum FT 12, were assessed for their antimicrobial properties against Porphyromonas gingivalis ATCC 33277 via disc diffusion assay. Anti-biofilm properties were determined by treating the overnight P. gingivalis ATCC 33277 biofilm with different concentrations of LAB cell-free supernatant (LAB CFS). Quantification of biofilm was carried out by measuring the optical density of stained biofilm. The ability of L. brevis FT 6 and L. plantarum FT 12 to tolerate salivary amylase was also investigated. Acid production with different sugars was carried out by pH measurement and screening for potential antimicrobial organic acid by disc diffusion assay of neutralised probiotics CFS samples. In this study, L. rhamnosus ATCC 7469, a commercial strain was used to compare the efficacy of the isolated strain with the commercial strain. Results: Lactobacillus brevis FT 6 and L. plantarum FT 12 possess antimicrobial activity against P. gingivalis with inhibition diameters of more than 10 mm, and the results were comparable with L. rhamnosus ATCC 7469. The MIC and MBC assay results for all tested strains were recorded to be 25 µl/µl concentration. All LAB CFS reduced biofilm formation proportionally to the CFS concentration and tolerated salivary amylase with more than 50% viability. Overnight cultures of all lactic acid bacteria strains showed a pH reduction and neutralised CFS of all lactic acid bacteria strains did not show any inhibition towards P. gingivalis. Conclusions: These results indicate that the isolated probiotics have the potential as probiotics to be used as a supportive oral health treatment, especially against a periodontal pathogen, P. gingivalis.en_US
dc.identifier.citationNurul Szawani Mohd-Zubri, Kalavathy Ramasamy, Nor Zaihana Abdul-Rahman, Characterization and potential oral probiotic properties of Lactobacillus plantarum FT 12 and Lactobacillus brevis FT 6 isolated from Malaysian fermented food, Archives of Oral Biology, Volume 143, 2022, 105515, ISSN 0003-9969, https://doi.org/10.1016/j.archoralbio.2022.105515en_US
dc.identifier.doi10.1016/j.archoralbio.2022.105515
dc.identifier.epage8
dc.identifier.issn0003-9969
dc.identifier.issue105515
dc.identifier.spage1
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9586
dc.identifier.volume143
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofARCHIVES OF ORAL BIOLOGYen_US
dc.subjectPeriodontal disease probiotic Phorpyromonas gingivalis lactic acid bacteria biofilmen_US
dc.titleCharacterization And Potential Oral Probiotic Properties Of Lactobacillus Plantarum Ft 12 And Lactobacillus Brevis Ft 6 Isolated From Malaysian Fermented Fooden_US
dc.typeArticleen_US
dspace.entity.typePublication

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