Effect of Friedelin Extract from Cannabis Roots on Cosmeceutical Application

Cannabis is a plant that has been used for several purposes from the past till now including leaves, flowers, stems, and roots. The cannabis root is not very popular because it does not contain cannabinoids. However, cannabis root contains the active ingredient in the triterpenoid group, friedelin. Many research had reported that friedelin can reduce inflammation and also has the ability of antibacterial. Thus, this research aimed to extract friedelin from cannabis roots and study comparative friedelin concentrations on inhibiting the growth of Propionibacterium acnes bacteria. Friedelin extracted from cannabis roots was tested for TLC to determine and analyze the primary purity of the resulting friedelin. Friedelin concentrations of 64, 256, and 1,024 micromolar were tested for inhibition of the growth of Propionibacterium acnes (P. acnes) bacteria by the disc diffusion method. The result of the TLC test friedelin from cannabis roots has a single component and a Rf value similar to the standard friedelin with distilled water can confirm that friedelin from cannabis roots is pure. For the result of inhibiting P. acnes bacteria of the Friedelin extract sample when compared with standard antibacterial clindamycin at a dose of 0.002 mg had a diameter of 48.34±2.16 mm in the P. acnes bacteria inactivation region but friedelin was ineffective with P. acnes bacteria, however, friedelin extract can be applied as a product to reduce acne in the form of reducing acne inflammation, but not to inhibit the growth of bacteria directly.