Publication:
The Relative Indirect Anthelmintic Effect of Caprine Milk on Mucins Gene Expression in Vitro Using IL-22 Treated LS174T Cells Model of Helminth Infection

Simple item page
dc.contributor.authorFadlul Azim Fauzi Mansuren_US
dc.contributor.authorAnis NA Raufen_US
dc.contributor.authorNur Fariha Mohd Manzoren_US
dc.contributor.authorFaizul Helmi Addnanen_US
dc.contributor.authorWan Omar Abdullahen_US
dc.contributor.authorOmaima A Najmen_US
dc.date.accessioned2024-05-27T14:35:00Z
dc.date.available2024-05-27T14:35:00Z
dc.date.issued2020
dc.date.submitted2022-1-3
dc.description.abstractMucus secretion by intestinal goblet cells constitutes an important mechanism in TH2 response following helminth infection mediated by the key cytokine IL-22. This indirect mechanism rather than directly attacking the parasite is important in preventing helminth attachment hence promoting helminth expulsion from the intestinal tract. We hypothesized that natural products having an anthelmintic activity like caprine milk may exert similar response. Using human intestinal LS174T cells treated with IL-22 to simulate helminth infection, we tested whether or not the co-treatment with caprine milk induces MUC1, MUC3, MUC4 and MUC5B genes expression. Optimal concentrations for caprine milk was determined to be 25% and 50% from cell viability assay. IL-22 induced helminth infection model was confirmed. However, the indirect anthelmintic effect of caprine milk was only relative as treatment of caprine milk in LS174T cells and IL-22 in vitro did not significantly induce MUC1, MUC3, MUC4 and MUC5B genes expression when compared to treatment with IL-22 alone. In conclusion, caprine milk was not significantly associated with the mechanism of increased mucus production through up regulation of mucin genes by intestinal cells. Caprine milk may possess direct anthelmintic effect rather than indirect.en_US
dc.identifier.citationFadlul A F Mansur, Anis NA Rauf, Nur FM Manzor, Faizul H Addnan, Wan O Abdullah, & Omaima A Najm. (2020). The Relative Indirect Anthelmintic Effect of Caprine Milk on Mucins Gene Expression in Vitro Using IL-22 Treated LS174T Cells Model of Helminth Infection. International Journal of Research in Pharmaceutical Sciences, 11((SPL 4), 1539–1545. Retrieved from https://ijrps.com/home/article/view/2207en_US
dc.identifier.epage1545
dc.identifier.issn0975-7538
dc.identifier.issueSPL4
dc.identifier.other819-27
dc.identifier.spage1539
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/3300
dc.identifier.volume11
dc.language.isoenen_US
dc.publisherJK Welfare & Pharmascope Foundationen_US
dc.relation.ispartofInternational Journal of Research in Pharmaceutical Sciencesen_US
dc.subjectanthelmintic, caprine milk, in vitro, IL-22, LS174Ten_US
dc.titleThe Relative Indirect Anthelmintic Effect of Caprine Milk on Mucins Gene Expression in Vitro Using IL-22 Treated LS174T Cells Model of Helminth Infectionen_US
dc.typeArticleen_US
dspace.entity.typePublication

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
274. The Relative Indirect Anthelmintic Effect Of Caprine Milk On Mucins Gene Expression In Vitro Using Il-22 Treated Ls174t Cells Model Of Helminth Infection.pdf
Size:
911.36 KB
Format:
Adobe Portable Document Format
Download

Collections

Other Publications