Publication: Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase
| dc.contributor.author | Zaidan, UH | en_US |
| dc.contributor.author | Rahman, MBA | en_US |
| dc.contributor.author | Othman, SS | en_US |
| dc.contributor.author | Basri, M | en_US |
| dc.contributor.author | Abdulmalek, E | en_US |
| dc.contributor.author | Rahman, RNZRA | en_US |
| dc.contributor.author | Salleh, AB | en_US |
| dc.date.accessioned | 2024-05-29T03:25:10Z | |
| dc.date.available | 2024-05-29T03:25:10Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | Enzyme immobilisation technology is an effective means to improve sugar ester production through the employment of biocatalysts. In the present study, immobilisation of Candida rugosa (CRL) lipase onto amino-activated mica is performed via covalent bonding (namely Amino-CRL) and the cross-linking of lipases into nano-reactors through physical adsorption (namely NER-CRL). Free and immobilised lipases were tested for their esterification activities. Specific activities for Amino-CRL and NER-CRL increased by 2.4 and 2.6-fold, respectively, upon immobilisation. Extending this work, immobilised lipases have novel capabilities in the synthesis of sugar esters. The optimised conditions for sugar fatty acid ester syntheses are 48 h at 2:1 of molar ratio of lactose sugar to capric acid at 55 C. Furthermore, a high operational stability with half-lives of over 13 and 10 runs was achieved for NER-CRL and Amino-CRL, respectively, indicating the efficiency of the immobilisation process. (C) 2011 Elsevier Ltd. All rights reserved. | |
| dc.identifier.doi | 10.1016/j.foodchem.2011.08.060 | |
| dc.identifier.epage | 205 | |
| dc.identifier.issn | 0308-8146 | |
| dc.identifier.issue | 1 | |
| dc.identifier.scopus | WOS:000296826400026 | |
| dc.identifier.spage | 199 | |
| dc.identifier.uri | https://oarep.usim.edu.my/handle/123456789/11892 | |
| dc.identifier.volume | 131 | |
| dc.language | English | |
| dc.language.iso | en_US | |
| dc.publisher | Elsevier Sci Ltd | en_US |
| dc.relation.ispartof | Food Chemistry | |
| dc.source | Web Of Science (ISI) | |
| dc.subject | Mica | en_US |
| dc.subject | Candida rugosa lipase | en_US |
| dc.subject | Covalent bonding | en_US |
| dc.subject | Cross-linking | en_US |
| dc.subject | Sugar esters | en_US |
| dc.title | Biocatalytic production of lactose ester catalysed by mica-based immobilised lipase | |
| dc.type | Article | en_US |
| dspace.entity.type | Publication |