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Embryo apoptosis identification: Oocyte grade or cleavage stage?

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dc.contributor.authorNoraina Mohd Bakrien_US
dc.contributor.authorSiti Fatimah Ibrahimen_US
dc.contributor.authorNurul Atikah Osmanen_US
dc.contributor.authorNurhaslina Hasanen_US
dc.contributor.authorFarah Hanan Fathihah Jaffaren_US
dc.contributor.authorZulaiha Abdul Rahmanen_US
dc.contributor.authorKhairul Osmanen_US
dc.date.accessioned2024-05-29T03:25:21Z
dc.date.available2024-05-29T03:25:21Z
dc.date.issued2016
dc.description.abstractApoptosis is a programed cell death that is vital for tissue homeostasis. However, embryo apoptosis had been known to be related to embryo fragmentation which should be avoided in in vitro fertilization (IVF). The purpose of this study was to evaluate the relationship of embryo apoptosis with the grade of immature oocytes and cleavage stage of in vitro produced (IVP) cattle embryos. This study consisted of 345 oocytes collected through ovary slicing. Immature oocytes were graded as A, B and C. This grading was based on cumulus cell thickness and compactness. All oocytes then underwent an in vitro maturation (IVM) procedure. An IVF was done 24 h after IVM culture. Prior to staining, stage of cleaved embryos was determined and classified as either 2, 4, 8 or > 8-cell embryo stage. Apoptosis status of cleaved IVP embryos was determined by using annexin V-FITC staining technique at 48 and 72 h post insemination (hpi). Apoptosis status for each embryo was classified as either early or late. The result showed that there was no significant difference (p > 0.05) of apoptosis status among grade A, B and C embryos. All grades of oocytes showed embryo apoptosis where 1.5% late apoptosis for grade A, 4.5% and 10.4% of early and late apoptosis for grade B and grade C. Early apoptosis was not seen in grade A embryo. We also noted no significant difference (p > 0.05) of apoptosis status between 2, 4, 8 and >8-cell embryo stage. Early apoptosis was also not seen in >8-cell stage. Even though there were no differences in apoptosis expression between the three classes, the cleavage rate of grade A oocytes was significantly higher (p < 0.01) than grade B and grade C. In conclusion, the apoptosis expression in the embryo can occur regardless of the oocyte quality and the cleavage stage of the embryo produced. (C) 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
dc.identifier.doi10.1016/j.sjbs.2015.10.023
dc.identifier.epageS55
dc.identifier.issn1319-562X
dc.identifier.issue1
dc.identifier.scopusWOS:000368475800008
dc.identifier.spageS50
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11946
dc.identifier.volume23
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofSaudi Journal Of Biological Sciences
dc.sourceWeb Of Science (ISI)
dc.subjectApoptosisen_US
dc.subjectOocyte gradingen_US
dc.subjectCleavage stageen_US
dc.titleEmbryo apoptosis identification: Oocyte grade or cleavage stage?en_US
dc.typeArticleen_US
dspace.entity.typePublication

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