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Green Synthesis, Characterization and Antibacterial Activity of Silver Nanoparticles-Kaempferol (Agnp-K) Towards Methicillin-Resistant Staphylococcus Aureus (Mrsa)

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datacite.subject.fosAgricultural sciences::Agricultural biotechnology
datacite.subject.fosNatural sciences::Earth and related Environmental sciences
dc.contributor.advisorRohazila Mohamad Hanafiah [Supervisor]
dc.contributor.authorAriff Haikal Bin Hairil Anuar
dc.date.accessioned2024-05-31T07:58:07Z
dc.date.available2024-05-31T07:58:07Z
dc.date.issued2024-05
dc.descriptionMatric: 3201480 (FPG)
dc.description.abstractThis study aims to characterize silver nanoparticles-kaempferol (AgNP-K) and its antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA). Nowadays, MRSA is a worldwide public health threat due to the resistance towards current antibiotics. Kaempferol was chosen due to its antibacterial capability. Green synthesis methods have been used to synthesize AgNP-K with the influence of temperature and different concentrations of silver nitrate and kaempferol (AgNO3:kaempferol). Sample C (AgNP-K 1:1 60/168) was synthesized with 1 mM concentration of kaempferol meanwhile sample Y (AgNP-K 1:2 60/168) with 2 mM of kaempferol. Characterization of sample C and sample Y were performed using UVVisible Spectroscopy (UV-Vis), zetasizer, transmission electron microscope (TEM), scanning electron microscope with a dispersive X-ray spectrometer (SEM-EDX), X-ray diffraction (XRD) and fourier transform infrared (FTIR). The presence of dark-brown colour in the solution indicated the formation of AgNP-K. UV-Visible absorption spectrum of the synthesized AgNP-K, sample C and sample Y showed a broad peak at range of 390 nm - 530 nm of wavelength. TEM, zetasizer and SEM-EDX results showed physical properties of sample C and sample Y such as morphology and size. The morphology analysis showed sample C and sample Y were in spherical shape. The size for sample C showed by zetasizer and TEM was 120.4 nm and 21.1487 ± 4.0183 nm respectively. Meanwhile, sample Y was 226.5 nm for zetasizer and 28.3604 ± 5.3418 nm in TEM. XRD analysis confirmed that AgNP-K was in crystalline phase structure, while FTIR showed (-OH) group absence in sample C and sample Y thus indicating that kaempferol was successfully incorporated with silver. Antibacterial activities of five samples (sample C, sample Y, commercial AgNP, kaempferol and vancomycin) with different concentrations (1.25, 2.5, 5, and 10 mg/mL) against MRSA were performed by using disc diffusion assay (DDA), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assay and time-kill assay. In DDA analysis, AgNP-K showed greater antibacterial activities against MRSA compared to kaempferol and commercial AgNP. The range values of MIC and MBC on both sample C and sample Y against MRSA were 1.25 mg/mL – 5 mg/mL, respectively. Time-kill assay analysis showed AgNP-K displayed bacteriostatic activity while bactericidal from MIC and MBC results towards MRSA. As conclusion, AgNP-K had been successfully synthesized from green synthesis method and performed characterization method by six (6) different instruments. From biological assay conducted showed that AgNP-K exhibited antibacterial activity against MRSA. As conclusions, silver nanoparticleskaempferol (AgNP-K), sample C exhibited better antibacterial activity when compared with sample Y kaempferol and commercial AgNP against MRSA.
dc.identifier.citationAriff Haikal Bin Hairil Anuar. (2024). Green Synthesis, Characterization and Antibacterial Activity of Silver Nanoparticles-Kaempferol (Agnp-K) Towards Methicillin-Resistant Staphylococcus Aureus (Mrsa) [Master’s thesis, Universiti Sains Islam Malaysia]. USIM Research Repository.
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/18832
dc.language.isoen_US
dc.subjectMethicillin-resistant Staphylococcus aureus (MRSA)
dc.subjectPublic health
dc.subjectKaempferol
dc.subjectGreen synthesis
dc.subjectUVVisible Spectroscopy (UV-Vis)
dc.subjectTransmission electron microscope (TEM)
dc.subjectScanning electron microscope with a dispersive X-ray spectrometer (SEM-EDX)
dc.subjectX-ray diffraction (XRD)
dc.subjectFourier transform infrared (FTIR)
dc.subjectDisc diffusion assay (DDA)
dc.subjectMinimum inhibitory concentration (MIC)
dc.subjectMinimum bactericidal concentration (MBC)
dc.subjectCharacterization and Antibacterial Activity of Silver Nanoparticles-Kaempferol (Agnp-K)
dc.subjectBiomass energy
dc.subjectGreen chemistry
dc.subjectBacteriology
dc.subjectNanochemistry
dc.subjectPlant anatomy
dc.titleGreen Synthesis, Characterization and Antibacterial Activity of Silver Nanoparticles-Kaempferol (Agnp-K) Towards Methicillin-Resistant Staphylococcus Aureus (Mrsa)
dc.typetext::thesis
dspace.entity.typePublication
oairecerif.author.affiliationUniversiti Sains Islam Malaysia

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