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The structure of a major surface antigen SAG19 from Eimeria tenella unifies the Eimeria SAG family

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dc.contributor.authorNur Zazarina Ramlyen_US
dc.contributor.authorSamuel R. Dixen_US
dc.contributor.authorSergey N. Ruzheinikoven_US
dc.contributor.authorSvetlana E. Sedelnikovaen_US
dc.contributor.authorPatrick J. Bakeren_US
dc.contributor.authorYock-Ping Chowen_US
dc.contributor.authorFiona M. Tomleyen_US
dc.contributor.authorDamer P. Blakeen_US
dc.contributor.authorKiew-Lian Wanen_US
dc.contributor.authorSheila Nathanen_US
dc.contributor.authorDavid W. Riceen_US
dc.date.accessioned2024-05-29T03:28:08Z
dc.date.available2024-05-29T03:28:08Z
dc.date.issued2021
dc.date.submitted2022-3-28
dc.description.abstractIn infections by apicomplexan parasites including Plasmodium, Toxoplasma gondii, and Eimeria, host interactions are mediated by proteins including families of membrane-anchored cysteine-rich surface antigens (SAGs) and SAG-related sequences (SRS). Eimeria tenella causes caecal coccidiosis in chickens and has a SAG family with over 80 members making up 1% of the proteome. We have solved the structure of a representative E. tenella SAG, EtSAG19, revealing that, despite a low level of sequence similarity, the entire Eimeria SAG family is unified by its three-layer αβα fold which is related to that of the CAP superfamily. Furthermore, sequence comparisons show that the Eimeria SAG fold is conserved in surface antigens of the human coccidial parasite Cyclospora cayetanensis but this fold is unrelated to that of the SAGs/SRS proteins expressed in other apicomplexans including Plasmodium species and the cyst-forming coccidia Toxoplasma gondii, Neospora caninum and Besnoitia besnoiti. However, despite having very different structures, Consurf analysis showed that Eimeria SAG and Toxoplasma SRS families each exhibit marked hotspots of sequence hypervariability that map to their surfaces distal to the membrane anchor. This suggests that the primary and convergent purpose of the different structures is to provide a platform onto which sequence variability can be imposed.en_US
dc.identifier.citationRamly, N.Z., Dix, S.R., Ruzheinikov, S.N. et al. The structure of a major surface antigen SAG19 from Eimeria tenella unifies the Eimeria SAG family. Commun Biol 4, 376 (2021). https://doi.org/10.1038/s42003-021-01904-wen_US
dc.identifier.doi10.1038/s42003-021-01904-w
dc.identifier.epage9
dc.identifier.issn2399-3642
dc.identifier.issue376
dc.identifier.other1002-7
dc.identifier.spage1
dc.identifier.urihttps://www.nature.com/articles/s42003-021-01904-w
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/12264
dc.identifier.volume4
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.ispartofCommunications Biologyen_US
dc.subjectParasite immune evasion, X-ray crystallographyen_US
dc.titleThe structure of a major surface antigen SAG19 from Eimeria tenella unifies the Eimeria SAG familyen_US
dc.typeArticleen_US
dspace.entity.typePublication

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