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Stemness and angiogenic gene expression changes of serial-passage human amnion mesenchymal cells

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dc.Chemicals/CAS5' nucleotidase, 9027-73-0; angiopoietin 1, 186270-49-5; endothelial nitric oxide synthase, 503473-02-7; fibroblast growth factor 2, 106096-93-9; glyceraldehyde 3 phosphate dehydrogenase, 9001-50-7; nestin, 146315-66-4; scatter factor, 67256-21-7, 72980-71-3; von Willebrand factor, 109319-16-6; Angiogenic Proteins; Antigens, Differentiation; Culture Media
dc.FundingDetailsUniversiti Kebangsaan Malaysia Ministry of Science, Technology and Space: 02-01-02-SF0289
dc.FundingDetailsThis study was funded by the Ministry of Science, Technology and Innovations , Grant number: 02-01-02-SF0289 . Simat Siti Fatimah is our PhD student in the Universiti Kebangsaan Malaysia and is a National Science Fellowship scholar of the Ministry of Science, Technology and Innovations. We would like to thank Sook Luan Ng for her technical help in neurogenic induction of HAMCs.
dc.MolecularSequenceNumbersGENBANK: NM_000442, NM_000552, NM_000603, NM_001146, NM_002006, NM_002007, NM_002046, NM_002253, NM_002632, NM_002701, NM_003106, NM_003508, NM_004334, NM_004827, NM_006617, NM_024865, NM_174900
dc.citedby30
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorFatimah S.S.en_US
dc.contributor.authorTan G.C.en_US
dc.contributor.authorChua K.en_US
dc.contributor.authorFariha M.M.N.en_US
dc.contributor.authorTan A.E.en_US
dc.contributor.authorHayati A.R.en_US
dc.date.accessioned2024-05-29T01:59:55Z
dc.date.available2024-05-29T01:59:55Z
dc.date.issued2013
dc.description.abstractBackground: Particular attention has been directed towards human amnion mesenchymal stem cells (HAMCs) due to their accessibility, availability and immunomodulatory properties. Therefore, the aim of the present study was to determine the temporal changes of stemness and angiogenic gene expressions of serial-passage HAMCs. Methods: HAMCs were isolated from human term placenta and cultured in serial passages in culture medium supplemented with 10% fetal bovine serum. Morphological analysis, growth kinetic and CFU-F assay of HAMCs were assessed. In vitro differentiation and the immunophenotype of HAMCs at P5 were also analyzed. Quantitative PCR was used to determine the stemness, angiogenic and endothelial gene expression of cultured HAMCs after serial passage. Results: Cultured HAMCs displayed intermediate epitheloid-fibroblastoid morphology at an initial culture and the fibroblastoid features became more pronounced in later passages. They showed high clonogenic activity and faster proliferation at later passages with colony forming efficiency of 0.88%. HAMCs were successfully differentiated into adipocytes, osteocytes and neuron-like cells. Most HAMCs expressed CD9, CD44, CD73, CD90 and HLA-A,B,C but negligibly expressed CD31, CD34, CD45, CD117 and HLA-DR,DP,DQ. After serial passage, stemness genes Oct-3/4, Sox-2, Nanog3, Rex-1, FGF-4 and FZD-9 expressions significantly decreased. Of the angiogenic genes PECAM-1, bFGF, eNOS, VEGFR-2, VEGF, and vWF expressions also decreased significantly except angiopoietin-1 which significantly increased. No significant differences were observed in ABCG-2, BST-1, nestin, PGF and HGF expressions after serial passage. Conclusion: These results suggested that cultured HAMCs could be an alternative source of stem cells and may have the potential for angiogenesis and hence its use in stem-cell based therapy. � 2012 Elsevier Inc.en_US
dc.description.natureFinalen_US
dc.identifier.CODENMIVRA
dc.identifier.doi10.1016/j.mvr.2012.12.004
dc.identifier.epage29
dc.identifier.issn262862
dc.identifier.issue1
dc.identifier.pmid23261754
dc.identifier.scopus2-s2.0-84873714138
dc.identifier.spage21
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84873714138&doi=10.1016%2fj.mvr.2012.12.004&partnerID=40&md5=a742f79cfe5ddbe0273283809e74e909
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10092
dc.identifier.volume86
dc.languageEnglish
dc.language.isoen_USen_US
dc.relation.ispartofMicrovascular Research
dc.sourceScopus
dc.titleStemness and angiogenic gene expression changes of serial-passage human amnion mesenchymal cellsen_US
dc.title.alternativeMicrovasc. Res.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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