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Modeling and Optimization of Lipozyme RM IM-Catalyzed Esterification of Medium- and Long-Chain Triacyglycerols (MLCT) Using Response Surface Methodology

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dc.FundingDetailsAcknowledgments Financial support for this work from Golden Hope Sdn. Bhd. is gratefully acknowledged. The authors would like to thank Mr. Azmi and Ms. Goh from MARDI for their kind technical assistance.
dc.citedby23
dc.contributor.affiliationsFaculty of Science and Technology
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsMalaysian Agricultural Research and Development Institute (MARDI)
dc.contributor.affiliationsSime Darby Research Sdn. Bhd.
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorArifin N.en_US
dc.contributor.authorSoo-Peng K.en_US
dc.contributor.authorLong K.en_US
dc.contributor.authorChin-Ping T.en_US
dc.contributor.authorYusoff M.S.A.en_US
dc.contributor.authorOi-Ming L.en_US
dc.date.accessioned2024-05-29T02:01:27Z
dc.date.available2024-05-29T02:01:27Z
dc.date.issued2012
dc.description.abstractOptimization conditions of Lipozyme RM IM lipase esterification of capric and stearic acids with glycerol for the production of medium- and long-chain triacyglycerols (MLCT) fat suitable for food applications such as margarine and shortening were investigated. Response surface methodology (RSM) was applied to model and optimize the reaction conditions, namely, the reaction time (8-24 h), enzyme load (5-15 Wt.%), and fatty acids/glycerol ratio (3:1-4:1) and represented by Ti, En, and Sb, respectively. Best-fitting models were successfully established for both MLCT yield (R 2 = 0.9507) and residual FFA (R 2 = 0.9315) established by multiple regressions with backward elimination. Optimal reaction conditions were 13.6-14.0 h for reaction time, 7.9-8.0 Wt.% for enzyme load, and 3:1 for fatty acids/glycerol molar ratio. Chi-square test showed that there were no significant (P>0.05) differences between the observed and predicted values of both models. Refined MLCT fat blend had sufficient solid fat at room temperature and made it suitable to use as a hard stock in shortening and margarine production. � 2010 Springer Science + Business Media, LLC.
dc.description.natureFinalen_US
dc.identifier.doi10.1007/s11947-010-0325-5
dc.identifier.epage225
dc.identifier.issn19355130
dc.identifier.issue1
dc.identifier.scopus2-s2.0-84856231200
dc.identifier.spage216
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84856231200&doi=10.1007%2fs11947-010-0325-5&partnerID=40&md5=aa24563de3383128a3f3a387a057749f
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10168
dc.identifier.volume5
dc.languageEnglish
dc.language.isoen_US
dc.relation.ispartofFood and Bioprocess Technology
dc.sourceScopus
dc.subjectEnzyme loaden_US
dc.subjectEsterificationen_US
dc.subjectFatty acids/glycerol molar ratioen_US
dc.subjectMedium- and long-chain triacyglycerols (MLCT)en_US
dc.subjectMLCT yielden_US
dc.subjectReaction timeen_US
dc.subjectResidual FFAen_US
dc.subjectResponse surface methodology (RSM)en_US
dc.subjectEnzyme loadsen_US
dc.subjectMLCT yielden_US
dc.subjectResidual FFAen_US
dc.subjectResponse Surface Methodologyen_US
dc.subjectTriacyglycerolsen_US
dc.subjectEnzymesen_US
dc.subjectEstersen_US
dc.subjectGlycerolen_US
dc.subjectHuman reaction timeen_US
dc.subjectOptimizationen_US
dc.subjectStatistical testsen_US
dc.subjectStearic aciden_US
dc.titleModeling and Optimization of Lipozyme RM IM-Catalyzed Esterification of Medium- and Long-Chain Triacyglycerols (MLCT) Using Response Surface Methodology
dc.typeArticleen_US
dspace.entity.typePublication

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