Publication:
In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia

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dc.ManufacturersHoffmann La Roche, Germany
dc.MolecularSequenceNumbersGENBANK: FJ948820, U39661
dc.TradenamesLightCycler 2.0, Hoffmann La Roche, Germany; LightCycler Probe Design 2.0, Hoffmann La Roche, Germany; RealTime ready RNA Virus Master kit, Hoffmann La Roche, Germany
dc.citedby4
dc.contributor.authorKen W.K.en_US
dc.contributor.authorRahman M.M.en_US
dc.contributor.authorHanafiah A.en_US
dc.contributor.authorIsahak I.en_US
dc.date.accessioned2024-05-29T01:55:43Z
dc.date.available2024-05-29T01:55:43Z
dc.date.issued2012
dc.description.abstractObjectives: Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children worldwide. Its detection by viral culture and immunofluorescent assay is time consuming and has low sensitivity. The objective of the study was to develop In House Light Cycler Real Time Polymerase Chain Reaction for Rapid Detection of Respiratory Syncytial Virus. Methodology: In house developed Light Cycler Real-Time reverse transcriptase polymerase chain reaction (LC-RT-PCR) assay was standardized using designed primers for the rapid identification of clinical samples of RSV. Results: A total of 130 samples (throat swab and nasopharyngeal aspirate) obtained from University Kebangsaan Malaysia Medical Centre, were efficiently tested and of which 54 (42%) were RSV positive. Conclusion: This study indicated that this newly developed in house rRT-PCR assay proved to be an excellent method for the rapid detection of RSV.en_US
dc.description.natureFinalen_US
dc.identifier.CODENPJMSC
dc.identifier.epage822
dc.identifier.issn1682024X
dc.identifier.issue5
dc.identifier.scopus2-s2.0-84873538712
dc.identifier.spage818
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84873538712&partnerID=40&md5=8174b4c5c0aca409efc40dd057649c33
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9743
dc.identifier.volume28
dc.languageEnglish
dc.language.isoen_USen_US
dc.relation.ispartofPakistan Journal of Medical Sciences
dc.sourceScopus
dc.subjectLightCycler real time polymerase chain reactionen_US
dc.subjectPrimeren_US
dc.subjectProbe and Plasmiden_US
dc.subjectRespiratory syncytial virusen_US
dc.subjectSequenceen_US
dc.titleIn-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysiaen_US
dc.title.alternativePak. J. Med. Sci.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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