Publication:
Evaluation Of Cholinesterase From The Muscle And Blood Of Anabas Testudineus As Detection Of Metal Ions

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dc.contributor.authorAhmad, SAen_US
dc.contributor.authorSabullah, MKen_US
dc.contributor.authorBasirun, AAen_US
dc.contributor.authorKhalid, Aen_US
dc.contributor.authorYasid, NAen_US
dc.contributor.authorIqbal, IMen_US
dc.contributor.authorShamaan, NAen_US
dc.contributor.authorSyed, MAen_US
dc.contributor.authorShukor, MYen_US
dc.date.accessioned2024-05-29T02:57:55Z
dc.date.available2024-05-29T02:57:55Z
dc.date.issued2016
dc.description.abstractAnother alternative source of cholinesterase (ChE) that is sensitive towards metal ion has been revealed. ChE from muscle and blood of Anabas testudineus were extracted and purified through ammonium sulphate precipitation followed by an ion exchange chromatography with a total recovery of 47.66% and 7.92%, respectively. Kinetic study measured that BTC was the most preferable synthetic substrate to blood ChE while muscle ChE preferred PTC with the biomolecular constant of 1.07 and 0.53 mM, respectively. Optimum pH for blood and muscle ChE were determined at 8 and 9. Both ChE shared an optimum temperature of 30 degrees C. Inhibition study showed that muscle ChE has inhibited more than 50% of metal ions namely arsenic, chromium, copper, mercury and zinc compared to blood ChE with only copper and mercury. Studies on half inhibitory effect (IC50) of blood and muscle ChE were tested with series concentration of mercury calculated at 1.003 and 1.048 mg/L. This result will be used as a reference for future development of biosensor.
dc.identifier.epage4260
dc.identifier.isbn1610-2304
dc.identifier.issn1018-4619
dc.identifier.issue10
dc.identifier.scopusWOS:000386746100050
dc.identifier.spage4253
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11663
dc.identifier.volume25
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletin
dc.sourceWeb Of Science (ISI)
dc.subjectCholinesteraseen_US
dc.subjectMetal ionsen_US
dc.subjectPurificationen_US
dc.subjectAnabas testudineusen_US
dc.titleEvaluation Of Cholinesterase From The Muscle And Blood Of Anabas Testudineus As Detection Of Metal Ionsen_US
dc.title.alternativeFresenius Environ. Bull.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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