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Pellet diameter of ganoderma lucidum in a repeated-batch fermentation for the trio total production of biomass-exopolysaccharide-endopolysaccharide and its anti-oral cancer beta-glucan response

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dc.contributor.authorAbdullah N.R.en_US
dc.contributor.authorSharif F.en_US
dc.contributor.authorAzizan N.H.en_US
dc.contributor.authorHafidz I.F.M.en_US
dc.contributor.authorSupramani S.en_US
dc.contributor.authorUsuldin S.R.A.en_US
dc.contributor.authorAhmad R.en_US
dc.contributor.authorWan-Mohtar W.A.A.Q.I.en_US
dc.date.accessioned2024-05-29T02:09:21Z
dc.date.available2024-05-29T02:09:21Z
dc.date.issued2020
dc.descriptionVolume :6 Issue :4en_US
dc.description.abstractThe pellet morphology and diameter range (DR) of Ganoderma lucidum were observed in a repeated-batch fermentation (RBF) for the trio total production of biomass, exopolysaccharide (EPS) and endopolysaccharide (ENS). Two factors were involved in RBF; broth replacement ratio (BRR: 60%, 75% and 90%) and broth replacement time point (BRTP: log, transition and stationary phase) in days. In RBF, 34.31 g/L of biomass favoured small-compact pellets with DR of 20.67 μm< d < 24.00 μm (75% BRR, day 11 of BRTP). EPS production of 4.34 g/L was prone to ovoid-starburst pellets with DR of 34.33 μm< d <35.67 μm (75% BRR, day 13 of BRTP). Meanwhile, the highest 2.43 g/L of ENS production favoured large-hollow pellets with DR of 34.00 μm< d < 38.67 μm (90% BRR, day 13 of BRTP). In addition, RBF successfully shortened the biomass-EPS–ENS fermentation period (31, 33 and 35 days) from batch to 5 days, in seven consecutive cycles of RBF. In a FTIR detection, β-glucan (BG) from EPS and ENS extracts were associated with β-glycosidic linkages (2925 cm-1, 1635 cm-1, 1077 cm-1,920 cm-1 and 800 cm-1 wavelengths) with similar1H NMR spectral behaviour (4.58, 3.87 and 3.81 ppm). Meanwhile, 4 mg/L of BG gave negative cytotoxic effects on normal gingival cell line (hGF) but induced antiproliferation (IC50 = 0.23 mg/mL) against cancerous oral Asian cellosaurus cell line (ORL-48). Together, this study proved that G. lucidum mycelial pellets could withstand seven cycles of long fermentation condition and possessed anti-oral cancer beta-glucan, which suits large-scale natural drug fermentation. © 2020 the Author(s), licensee AIMS Press.en_US
dc.identifier.doi10.3934/microbiol.2020023
dc.identifier.epage400
dc.identifier.issn2471-1888
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85095834353
dc.identifier.spage379
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85095834353&doi=10.3934%2fmicrobiol.2020023&partnerID=40&md5=e6dac53758558105d3c83d21c6194074
dc.identifier.urihttps://www.aimspress.com/article/doi/10.3934/microbiol.2020023
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10460
dc.identifier.volume6
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherAIMS Pressen_US
dc.relation.ispartofAdvances in Intelligent Systems and Computingen_US
dc.sourceScopus
dc.subjectAnti-oral canceren_US
dc.subjectEndopolysaccharideen_US
dc.subjectExopolysaccharideen_US
dc.subjectGanoderma lucidumen_US
dc.subjectPellet morphologyen_US
dc.subjectRepeated-batch fermentationen_US
dc.titlePellet diameter of ganoderma lucidum in a repeated-batch fermentation for the trio total production of biomass-exopolysaccharide-endopolysaccharide and its anti-oral cancer beta-glucan responseen_US
dc.typeArticleen_US
dspace.entity.typePublication

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