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Antimicrobial Protein Produced By Lactobacillus Plantarum Atcc8014 In The Presence Of Cymbopogon Sp. Essential Oils

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dc.contributor.authorHanina Mohd Nooren_US
dc.contributor.authorHairul Shahril Muhamaden_US
dc.contributor.authorIsmatul Nurul Asyikin Ismailen_US
dc.contributor.authorA’firah Mohd Sakri Shukrien_US
dc.contributor.authorSalina Mat Radzien_US
dc.contributor.authorMaryam Mohamed Rehanen_US
dc.contributor.authorAbdul Jalil Abdul Kaderen_US
dc.date.accessioned2024-05-28T03:28:06Z
dc.date.available2024-05-28T03:28:06Z
dc.date.issued2015
dc.date.submitted1/9/2020
dc.descriptionApplied Mechanics and Materials (Volumes 754-755)en_US
dc.description.abstractProtein production by bacteria might be increased in stressful conditions such as in the presence of antimicrobial agents. Many studies proved that antibiotics or antimicrobial agents at low concentration are able to activate or repress gene transcription process in bacteria. However, there are still few studies on potential of natural antimicrobial compounds such as Cymbopogon essential oils acting as specific chemical signal that can trigger biological functions of bacteria. Therefore, this study aims to explore the potential of natural antimicrobial compound (Cymbopogon flexuosus and Cymbopogon nardus) at low concentration in regulating proteins production by Lactobacillus plantarum ATCC8014. The bacteria cells of L. plantarum ATCC8014 are exposed to Cymbopogon essential oils at low concentration in fermentation process for 48 hours at 37°C. SDS-PAGE analysis showed that a new intracellular protein with approximate size of 40 kDa was produced by L. plantarum ATCC8014 after being enhanced with C. nardus essential oil. Besides, the intracellular proteins, each with approximate size of 85 kDa, 45 kDa and 28 kDa synthesized by L. plantarum ATCC8014 prior to inducing with C. nardus or C. flexuosus were expressed differently. Some of the intracellular proteins were highly expressed and some of the proteins were repressed based on the intensity of protein bands appeared. Hence, L. plantarum ATCC8014 in the presence of Cymbopogon essential oils at low concentration could regulate the intracellular proteins production. The isolated protein also showed antimicrobial activity against selected Gram-positive and Gram-negative bacteria.en_US
dc.identifier.citationMohd Noor, Hanina, Hairul Shahril Muhamad, Ismatul Nurul Asyikin Ismail, A’firah Mohd Sakri Shukri, Salina Mat Radzi, Maryam Mohamed Rehan, and Abdul Jalil Abdul Kader. “Antimicrobial Protein Produced by LactobaCillus pLantarum ATCC8014 in the Presence of Cymbopogon Sp. Essential Oils.” Applied Mechanics and Materials. Trans Tech Publications, Ltd., April 2015. https://doi.org/10.4028/www.scientific.net/amm.754-755.1045.en_US
dc.identifier.doi10.4028/www.scientific.net/AMM.754-755.1045
dc.identifier.epage1053
dc.identifier.issn1660-9336
dc.identifier.issue5
dc.identifier.other2387-9
dc.identifier.spage1045
dc.identifier.urihttps://www.scientific.net/AMM.754-755.1045
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/4239
dc.identifier.volume754-755
dc.language.isoenen_US
dc.publisherTrans Tech Publications, Switzerlanden_US
dc.relation.ispartofApplied Mechanics and Materialsen_US
dc.subjectAntimicrobial Protein,en_US
dc.subjectCymbopogon sp. Essential Oils,en_US
dc.subjectLactobacillus plantarum ATCC8014en_US
dc.titleAntimicrobial Protein Produced By Lactobacillus Plantarum Atcc8014 In The Presence Of Cymbopogon Sp. Essential Oilsen_US
dc.typeArticleen_US
dspace.entity.typePublication

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